Project description:Purpose: Examining the transcriptome of human gut bacteria that grow on seaweed polysaccharides as a sole carbon source Methods: Strains were grown on 5 mg/ml seaweed polysaccharides (carrageenan, agarose and/or poprhyran respective to strain) or galactose as a sole carbon source in vitro. Fold change was calculated as seaweed polysaccharide over galactose with n=2 biological replicates. Once cells reached an optical density corresponding to mid-log phase growth, RNA was isolated and rRNA depleted. Samples were multiplexed for sequencing on the Illumina HiSeq platform at the University of Michigan Sequencing Core. Data was analyzed using Arraystar software (DNASTAR, Inc.) Genes with significant up- or down-regulation were determined by the following criteria: genes with an average fold-change >10-fold and with both biological replicates with a normalized expression level >1% of the overall average RPKM expression level. READS WERE ANALYZED .......GABRIEL FILL IN Results: We identified novel polysaccharide utiilization loci in 5 strains of human gut bacteria
Project description:This study aimed at identification of genetic regulations for desiccation tolerance in intertidal seaweed species Ulva lactuca most commonly experienced phenomenon of intertidal communities.
Project description:The aim of this study was to elucidate which changes in gene expression are related to the induced anti-herbivory response in the brown seaweed Fucus vesiculosus. Microarray hybridizations were used to identify genes differentially expressed in response to grazing by the isopod Idotea baltica. This study shows that modifications in gene expression occur simultaneously to a reduced palatability and that defence induction entails reallocation of resources from primary to secondary metabolism with down-regulation of photosynthesis and degradation of storage compounds.
Project description:Columbia Arabidopsis ecotype were grown hydroponically on 1 mM NH4NO3 as sole nitrogen source during 35 days under short days. Plants leaves were then sprayed with a seaweed extract or with water (control). Root and shoot samples were harvested separately 1 hour and 7 days after this spraying.
Project description:This study aimed at identification of genetic regulations for desiccation and submergence tolerance in intertidal seaweed species Ulva lactuca which arisies periodically due to tidal rhythms.
