Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MiRNA-seq of HEK293 cell lines on Illumina iSeq100 instrument

ABSTRACT: miRNA-seq of HEK293 cell lines on Illumina iSeq100 instrument

PROVIDER: PRJEB62424 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR11452628.fastq.gz	Fastqsanger.gz
	ERR11452629.fastq.gz	Fastqsanger.gz
	ERR11452630.fastq.gz	Fastqsanger.gz
	ERR11452631.fastq.gz	Fastqsanger.gz
	ERR11452632.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

miRNA-seq of HEK293 cell lines on Illumina iSeq100 instrument

Project description:A validation experiment performed on HEK293 cell lines after genome editing. The design contains three duplicate runs consisted of: HEK293 wild type cell line HEK293 with MIR484 gene knockdown using CRISPR-Cas9 HEK293 with MIR185 gene knockout using CRISPR-Cas9

2023-06-16 | E-MTAB-13009 | biostudies-arrayexpress

NPAS3-HEK293-standardcond-Illumina

Project description:NPAS3 or truncated NPAS3 overexpressed HEK293 cells or control HEK293 cells were collected at 24 hours after gene overexpression.Total RNA from alll samples was extracted.Biotinylated cRNA was prepared using the Illumina total Prep RNA application Kits.Hybridization, washing and scanning were performed according to the Illumina BeadStation 500* manual (revision C)

2011-10-03 | E-TABM-1078 | biostudies-arrayexpress

NPAS3-HEK293-circadian-Illumina

Project description:The fragments of FLNPAS3 and DeltaNPAS3 were transferred into a similar TET-inducible expression plasmid and transfected into HEK293 [T-REx-293] cells (Invitrogen). At the zero hour time-point, cells were shifted into a serum-rich medium plus tetracycline in order to induce circadian cycling and NPAS3 overexpression. Parental HEK293 [T-REx-293] cells after circadian rhythmcity induction for +12hr/+24hrs were used as negative controls because stably integrated FLNPAS3/DeltaNPAS3 cell lines showed a low level of leaky transcription in the absence of tetracycline. At +2 hours, cells were washed with DMEM and then incubated with the same plus tetracycline for the remaining period of the experiment. Cell samples were collected at +12hrs and +24hrs respectively. For all circadian cell culture experiments with FLNPAS3/DeltaNPAS3/parental negative control cell lines, duplicate biological samples were assessed. Microarray probes synthesised from RNA extraction products was quantified using an Agilent Bioanalyzer to ensure high quality probes of equal quantity between experiments. Sentrix® HumanRef-8 v2 chips were used to detect gene expression profiles.

2011-10-03 | E-TABM-1079 | biostudies-arrayexpress

HEK293 cells 100 cell RNAseq profiling on Illumina NextSeq 500

Project description:Three libraries from 100 HEK293 cells each were prepared using a Smartseq based custom library preparation approach with unique molecular identifiers. Libraries were sequenced on a Illumina NextSeq 500

2016-12-19 | GSE79133 | GEO

microRNA sequencing of HEK293 and H9c2 cells

Project description:The human HEK293 / 293T and rat cardiomyoblast H9c2 cell lines are commonly employed for microRNA-mRNA interaction studies. Here, I provide microRNA sequencing data obtained from each of these lines to better document which microRNAs are endogenously expressed at high or low levels. Small RNA sequencing profiles were generated from cultured HEK293 and H9c2 cells on Illumina HiSeq 2000 instruments.

2014-09-26 | E-GEOD-61770 | biostudies-arrayexpress

Analysis of M229-Par and M229-Res cells by Illumina HiSeq instrument (4000 or equivalent) seq analysis .

Project description:Analysis of M229-Par and M229-Res cells by Illumina HiSeq instrument (4000 or equivalent) seq analysis .

| PRJNA834743 | ENA

bulk RNA-seq analysis comparing HEK293 and HBEC 5i cell lines

Project description:bulk RNA-seq analysis comparing HEK293 and HBEC 5i cell lines

| PRJNA612700 | ENA

Chordoma and osteosarcoma cell lines Illumina Infinium MethylationEPIC array

Project description:Chordoma and osteosarcoma cell lines Illumina Infinium MethylationEPIC array

| PRJNA579571 | ENA

Equine tissue miRNA profiling

Project description:Purpose: Identification of miRNA expression profiles of selected horse tissues. Methods: miRNA-seq analysis was performed on lung, heart and liver samples collected from 4 horses. The miRNA libraries were constructed from total RNA using NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs) according to the manufacturer protocol. The quantification of the obtained libraries was performed on a Qubit 2.0 spectrophotometer (Invitrogen, Life Technologies), while a quality control on a TapeStation 2200 instrument (D1000 ScreenTape; Agilent). 75 single-end cycle sequencing was performed on the NextSeq 500/550 platform (Illumina) with the use of NextSeq High Output Reagents v2 (Illumina). Results: The comparison of miRNA profiles between the investigated tissues revealed 185 differentially expressed microRNAs (p adj≤0.05) in the heart samples versus the liver samples, 172 DE miRNAs (p adj ≤0.05) in the lung samples in comparison to the liver samples, and 155 in the lung samples versus the heart samples. Conclusions: Obtained results show varied miRNA expression profiles characteristics for each investigated hore tissue.

2023-06-01 | GSE202502 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and RBM7-/- HEK293 cell lines Transcriptomes and RBM7 targetted RNAs

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived transcriptome profiling of Wild Type and RBM7-/- HEK293 cell lines and RNA immunoprecipitation (RIP) of Flag-tagged RBM7-expressing HEK293 cells

2020-08-31 | GSE144075 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data