Project description:RNA was extracted from FFPE samples by manual microdissection of the tumor zones. RNAseq libraries were perfomed using Lexogen's QuantSeq (reverse) 3' kit
Project description:We profiled the transcriptomes of the dominan nodule (Gleason score 4+5=9) and of the cancer free resection margins of a surgically removed prostate tumor from a 65y old black man. Normalized to the median of valid spots expression data where used to determine the Gene Commanding Height (GCH) Score for every coding and non-coding transcript adequately quantified in all four cancer and four normal tissue samples. The analysis proved that cancer and normal tissue have distinct gene hiearchies. The top three genes in the cancer region were: BAIAP2L1 (BAI1-associated protein 2-like 1 ), FAM71E1 (amily with sequence similarity 71, member E1 ) and MAP6D1 (MAP6 domain containing 1 ), while the top three genes in the cancer-free region were: TOR1A (torsin family 1, member A ), MRPS12 (mitochondrial ribosomal protein S12 ) and GTF2H1 (general transcription factor IIH, polypeptide 1).
Project description:We profiled the transcriptomes of the cancer nucleus (Gleason score 4+5=9) and of the cancer free resection margins of a surgically removed prostate tumor from a 47y old white man. Normalized to the median of valid spots expression data where used to determine the Gene Commanding Height (GCH) Score for every coding and non-coding transcript adequately quantified in all four cancer and four normal tissue samples. The analysis proved that cancer and normal tissue have distinct gene hiearchies. The top three genes in the cancer region were: LOC145474 (uncharacterized long non-coding RNA), PRRG1 (proline rich Gla (G-carboxyglutamic acid) 1) and ASAP3 (ArfGAP with SH3 domain, ankyrin repeat and PH domain 3), while the top three genes in the cancer-free region were: SFR1 (SWI5-dependent recombination repair 1 ), EDF1 (endothelial differentiation-related factor 1) and RHOD (ras homolog family member D).
Project description:Gene Commanding Height (GCH) hierarchy in the cancer nucleus and cancer-free resection margins from a surgically removed prostatic adenocarcinoma of a 47y old white man
Project description:Gene Commanding Height (GCH) hierarchy in the cancer nucleus and cancer-free resection margins from a surgically removed prostatic adenocarcinoma of a 65y old black man
Project description:RATIONALE: Chemoprevention therapy is the use of certain drugs to try to prevent the development or recurrence of cancer. The use of folic acid may be an effective way to prevent colorectal cancer in patients with polyps that have been surgically removed.
PURPOSE: Randomized phase II trial to study the effectiveness of high-dose folic acid in preventing colorectal cancer in patients who have had polyps surgically removed within 18 months of the trial.
Project description:RATIONALE: Chemoprevention therapy is the use of certain drugs to try to prevent the development or recurrence of cancer. The use of aspirin may be an effective way to prevent the recurrence of polyps in colorectal cancer.
PURPOSE: Randomized phase III trial to study the effectiveness of aspirin in treating patients who have stage I, stage II, or stage III colorectal cancer that has been surgically removed.
Project description:RATIONALE: Drugs used in chemotherapy use different ways to stop tumor cells from dividing so they stop growing or die.
PURPOSE: Phase II trial to study the effectiveness of capecitabine in treating older patients who have metastatic or recurrent colorectal cancer that cannot be surgically removed.