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RNA-seq of MCF10A transduced with an inducible lentiviral MYB expression system

ABSTRACT: RNA-seq of MCF10A transduced with an inducible lentiviral MYB expression system

PROVIDER: PRJEB62551 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR11475010_1.fastq.gz	Fastqsanger.gz
	ERR11475010_2.fastq.gz	Fastqsanger.gz
	ERR11475011_1.fastq.gz	Fastqsanger.gz
	ERR11475011_2.fastq.gz	Fastqsanger.gz
	ERR11475012_1.fastq.gz	Fastqsanger.gz

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RNA-seq of MCF10A transduced with an inducible lentiviral MYB expression system

Project description:The non-tumourigenic breast cell line MCF10A was transduced using pINDUCER21-MYB vector to express MYB upon addition of doxyclyclin (DOX), and compared to an empty vector (EV) control (pINDUCER21 (ORF-EG)) with and without the addition of DOX

2023-12-01 | E-MTAB-12977 | biostudies-arrayexpress

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Expression data of cultured MCF10A cells transduced with MYB or MYB-NFIB expression vectors

Project description:This experiment investigates differences between control cells (empty vector) and cells with MYB or MYB-NFIB (M14N9) overexpression using MSCV vectors

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Project description:Human Burkitt's lymphoma ST486 cells were transduced with non-target control shRNA lentiviral vectors, FOXM1 shRNA, and MYB shRNA lentiviral vectors. Total RNA was isolated 24h later. cRNA was produced with the standard one-step IVT protocol (Affymetix) and hybridized in U95Av2 gene chips (Affymetrix). Experiment consists in 3 independent samples: Expression profiling of Burkitt's lymphoma cells 24h after non-target control shRNA lentiviral mediated transduction. Expression profiling of Burkitt's lymphoma cells 24h after FOXM1 shRNA lentiviral mediated transduction. Expression profiling of Burkitt's lymphoma cells 24h after MYB shRNA lentiviral mediated transduction. Data processing performed using MAS5 or GCRMA.

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