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IlLeuComm

ABSTRACT: Leucania comma, genomic and transcriptomic data

PROVIDER: PRJEB62566 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
	ERR11483517.fastq.gz	Fastqsanger.gz
	ERR11496086_1.fastq.gz	Fastqsanger.gz
	ERR11496086_2.fastq.gz	Fastqsanger.gz
	ERR12861035_1.fastq.gz	Fastqsanger.gz
	ERR12861035_2.fastq.gz	Fastqsanger.gz

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Project description:This gene expression microarray analysis is to test how induced Bcl11b expression affect the overall gene expression profile of Comma D Beta Cells in the progenitor (sca1+) and differentiated (Sca1-) population. Comma D beta Cell line was cultured DMEM F12+2% FBS+1% PSA+5ug/ml insulin+10ng/ml EGF in 175 cm2 culture flask overnight, followed by 100ng/ml Doxycycline treatment for 1 day. Then, cells were trypsinized and subjected for flow sorting of Sca1+ and Sca- population. RNA was then extracted and subjected to microarray gene expression analysis. Sca1+ and Sca1- cells in the presence or absence of Doxycycline.

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