Soil microbial community (Polmak 2015 exclosure experiment)
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ABSTRACT:
PROVIDER: PRJEB62634 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:A small, shed antler fragment of a reindeer from Sjælland, Denmark has been dated to the Mid-Holocene, ca., 4700 cal B.C. Reindeer was an important component of the Lateglacial fauna in Denmark, and the species survived for ca. 1400 years into the Holocene. However, we consider it highly unlikely that this species inhabited Denmark during the Mid-Holocene, when dense forests characterized the vegetation and summer temperatures were somewhat higher than at present. We suggest that the reindeer antler came to Sjælland from Norway or Sweden as a result of trade, perhaps involving flint.
2021-11-25 | PXD024143 | Pride
Project description:The expression of stress-related genes induced by feeding of chestnut moth larvae (Conistra vaccinii L.) was studied with Vitreoscilla hemoglobin-expressing (VHb) and non-transgenic hybrid aspen lines (Populus tremula x P. tremuloides). Besides the herbivore-injured leaves (L1), cDNA microarray analyses were conducted using uninjured leaves of hybrid aspen lines positioned above (A) and below (B) the herbivory exposed leaves.
2013-04-24 | GSE46331 | GEO
Project description:Arabidopsis thaliana plants (ecotype Landsberg erecta) were grown at 20˚C constant temperature, 8 hr/16 hr Light/Dark photoperiod at 50-60% ambient humidity, for 8 to 9 weeks. Short day conditions prevented the onset of flowering and the plants were thus maintained in growth stage 1 (leaf production) with 13 to 15 rosette leaves larger than 1mm (stage 1.13 to 1.14). Diamondback moth (DBM, Plutella xylostella) larvae were maintained on cabbage (Brassica oleracea) plants in a climate-controlled room at 25ºC, 12 hr photoperiod with 50%-60% relative humidity. Two days before exposing A. thaliana plants to herbivore treatment, plants were transferred to a climate-controlled room (22ºC, 50-60% humidity, 12 hr photoperiod). For insect treatment, seven Diamondback moth (DBM, Plutella xylostella) larvae (third to fifth instars) were placed on a group of four or five plants until time of harvest, for each time point separately. All rosette leaves were harvested at 1h, 4h, 12h, and 24h after onset of continuous herbivory and flash frozen in liquid nitrogen. Control plants were maintained under the same conditions and harvested in parallel. Keywords: time course, stress response, herbivory response
2008-03-04 | GSE10681 | GEO
Project description:The aim of this study was to elucidate which changes in gene expression are related to the induced anti-herbivory response in the brown seaweed Fucus vesiculosus. Microarray hybridizations were used to identify genes differentially expressed in response to grazing by the isopod Idotea baltica. This study shows that modifications in gene expression occur simultaneously to a reduced palatability and that defence induction entails reallocation of resources from primary to secondary metabolism with down-regulation of photosynthesis and degradation of storage compounds. Total RNA of Fucus vesiculosus pieces that were or were not previously grazed by Idotea baltica for 15 and 18 days (3 biological replicates each) was hybridized against pooled RNA from 4 F. vesiculosus pieces that were collected before onset of grazing (day 0).
2013-06-19 | E-GEOD-47975 | biostudies-arrayexpress
Project description:The aim of this study was to elucidate changes in gene expression related to the induced anti-herbivory response in the brown seaweed Fucus vesiculosus. Microarray hybridizations were used to identify genes differentially expressed in response to grazing by the periwinkle Littorina obtusata. This study shows that modifications in gene expression occur simultaneously to a reduced palatability and that defence induction entails reallocation of resources from primary to secondary metabolism with down-regulation of photosynthesis. Total RNA of Fucus vesiculosus pieces that were or were not previously grazed by Littorina obtusata for 3, 12, 15, 21, and 24 days (5 biological replicates each) was hybridized against pooled RNA from 5 F. vesiculosus pieces that were collected before onset of grazing (day 0).
2013-12-13 | E-GEOD-53262 | biostudies-arrayexpress
Project description:The expression of stress-related genes induced by feeding of chestnut moth larvae (Conistra vaccinii L.) was studied with Vitreoscilla hemoglobin-expressing (VHb) and non-transgenic hybrid aspen lines (Populus tremula x P. tremuloides). Besides the herbivore-injured leaves (L1), cDNA microarray analyses were conducted using uninjured leaves of hybrid aspen lines positioned above (A) and below (B) the herbivory exposed leaves. Two-condition experiment, control vs. herbivory exposure. Two hybrid aspen lines: non-transgenic V617 and VHb expressing V617 /45. Of each plant, three leaf types were analysed: the injured/uninjured leaf (L1) and nonorthostichous leaf positioned above (A) and below (B). Biological replicates: 3. On each array, two samples representing L1, A or B leaf type of control and herbivory treatment of either V617 or V617/45 line. line V617: wt_A_rep1-3, wt_B_rep1-3, wt_L1_rep1-3 line V617/45: VHb_A_rep1-3, VHb_B_rep1-3, VHb_L1_rep1-3 leaf type A: wt_A_rep1-3, VHb_A_rep1-3 leaf type B: wt_B_rep1-3, VHb_B_rep1-4 leaf type L1: wt_L1_rep1-3, VHb_L1_rep1-5
2013-04-24 | E-GEOD-46331 | biostudies-arrayexpress
Project description:The aim of this study was to elucidate changes in gene expression related to the induced anti-herbivory response in the brown seaweed Fucus vesiculosus. Microarray hybridizations were used to identify genes differentially expressed in response to grazing by the periwinkle Littorina obtusata. This study shows that modifications in gene expression occur simultaneously to a reduced palatability and that defence induction entails reallocation of resources from primary to secondary metabolism with down-regulation of photosynthesis.
2013-12-13 | GSE53262 | GEO
Project description:UV-B radiation regulates numerous morphogenic, biochemical and physiological responses in plants, and can stimulate some responses typically associated with other abiotic and biotic stimuli, including invertebrate herbivory. Removal of UV-B from the growing environment of various plant species has been found to increase their susceptibility to consumption by invertebrate pests, however, to date, little research has been conducted to investigate the effects of UV-B on crop susceptibility to field pests. Here, we report findings from a multi-omic and genetic-based study investigating the mechanisms of UV-B-stimulated resistance of the crop, Brassica napus (oilseed rape), to herbivory from an economically important lepidopteran specialist of the Brassicaceae, Plutella xylostella (diamondback moth). The UV-B photoreceptor, UV RESISTANCE LOCUS 8 (UVR8), was not found to mediate resistance to this pest. RNA-Seq and untargeted metabolomics identified components of the sinapate/lignin biosynthetic pathway that were similarly regulated by UV-B and herbivory. Arabidopsis mutants in genes encoding two enzymes in the sinapate/lignin biosynthetic pathway, CAFFEATE O-METHYLTRANSFERASE 1 (COMT1) and ELICITOR-ACTIVATED GENE 3-2 (ELI3-2), retained UV-B-mediated resistance to P. xylostella herbivory. However, overexpression of B. napus COMT1 in Arabidopsis further reduced plant susceptibility to P. xylostella herbivory in a UV-B-dependent manner. These findings demonstrate that overexpression of a component of the sinapate/lignin biosynthetic pathway in a member of the Brassicaceae can enhance UV-B-stimulated resistance to herbivory from P. xylostella.
2023-08-01 | GSE239314 | GEO
Project description:The aim of this study was to elucidate which changes in gene expression are related to the induced anti-herbivory response in the brown seaweed Fucus vesiculosus. Microarray hybridizations were used to identify genes differentially expressed in response to grazing by the isopod Idotea baltica. This study shows that modifications in gene expression occur simultaneously to a reduced palatability and that defence induction entails reallocation of resources from primary to secondary metabolism with down-regulation of photosynthesis and degradation of storage compounds.
2013-06-19 | GSE47975 | GEO