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PREDICT

ABSTRACT: PREDICT: Omics-based models for prediction of hybrid performance in oilseed rape

PROVIDER: PRJEB63226 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR11600100.fastq.gz	Fastqsanger.gz
	ERR11600200.fastq.gz	Fastqsanger.gz
	ERR11600300.fastq.gz	Fastqsanger.gz
	ERR11600400.fastq.gz	Fastqsanger.gz
	ERR11600500.fastq.gz	Fastqsanger.gz

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Leptosphaeria maculans gene expression during in vitro growth and primary oilseed rape leaf infection

Project description:The transcriptome of Leptosphaeria maculans was analyzed in mycelium and during oilseed rape (Brassica napus) leaf infection. The array probes were designed from gene models from the L. maculans whole genome annotation. One aim of this study was to verify the expression of the automatically annotated gene models in various conditions. Another goal was to monitor gene expression profiles during oilseed rape leaf infection and to highlight tissue-specific transcripts, e.g. in plant up-regulated transcripts, for further analyses. We performed 9 hybridizations (NimbleGen) with samples derived from mycelium and infected oilseed rape leaves. Samples from infected oilseed rape leaves were harvested 7 and 14 days post infection. Three replicates each. All samples were labeled with Cy3.

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