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VIDI_14854_bsa_01_brassica_nigra

ABSTRACT: Genetic mapping through BSA-seq of Pieris egg-induced HR in Brassica nigra

PROVIDER: PRJEB64240 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR11771286_1.fastq.gz	Fastqsanger.gz
	ERR11771286_2.fastq.gz	Fastqsanger.gz
	ERR11771287_1.fastq.gz	Fastqsanger.gz
	ERR11771287_2.fastq.gz	Fastqsanger.gz
	ERR11771288_1.fastq.gz	Fastqsanger.gz

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Brassica nigra challenged by Pieris brassicae, eggs of Pieris brassicae, Xanthomonas campestris pv. raphani, Brevicoryne brassicae or by combined stresses eggs of P. brassicae / P. brassicae, X. campestris / P. brassicae, B. brassicae / P. brassicae

Project description:Brassica nigra plants, a Brassicaceae close to Arabidopsis thaliana, was used for combined stresses experiments. In this study, we performed a whole-genome microarray analysis on five-week-old plants and compared untreated plants and plants treated different single or dual stresses: the larvae Pieris brassicae, egg extract of Pieris brassicae, the bacterial Xanthomonas campestris pv. raphani, the aphid Brevicoryne brassicae or by combined stresses eggs of P. brassicae / P. brassicae, X. campestris / P. brassicae, B. brassicae / P. brassicae.

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Pieris brassicae gut

Project description:Pieris brassicae egg/Brassica nigra leaf 16S metabarcoding reads

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Brassica nigra challenged by ozone, Pieris brassicae and both ozone followed by P. brassicae feeding.

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Project description:GC-TOF-MS, Brassica nigra / MeJA + herbivory

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RNA-Seq of Arabidopsis thaliana and Arabidopsis lyrata plants primed by insect eggs from Pieris brassicae and triggered by insect herbivory

Project description:In Arabidopsis thaliana it is known that plants that harboured eggs of the White cabbage butterfly (Pieris brassicae) before larval feeding can defend better against the herbivore stress. As Arabidopsis thaliana is an annual plant we were interested in the plant defense and possible priming reaction of the closest biannual/perennial relative Arabidopsis lyrata. The main aim of the experiment was to compare the effect induced by insect egg deposition of Pieris brassicae between A. thaliana and A. lyrata plants grown in parallel on the transcriptional level. We used a full factorial setup consisting of a) untreated control plants , b) plants which experienced eggs for 6 days without larval feeding after that period, c) plants which experienced no eggs before larval feeding for 24 hours d) plants which experienced eggs for 6 days and larval herbivory for 24 hours. This setup was conducted with 7 week old vegetative plants For all treatments leaf tissue from the leaves that experienced egg oviposition and/or larval feeding were collected.

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Project description:Fine mapping of Brwax through BSA-seq

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Project description:Fine mapping of Brwf4 through BSA-seq

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Transcription profiling of Arabidopsis local and distal leaves in response to eggs of the Pieris rapae butterfly using leaf disks the size of the egg batch

Project description:Expression profiling of the affect of eggs of the Pieris brassicae butterfly on Arabidopsis thaliana local leaf vs. the distal leaf using leaf disks the size of the egg batch.

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Gene expression in Arabidopsis leaves after P. brassicae egg deposition and/or herbivory by P. brassicae larvae

Project description:Plants can prepare their defense of impending herbivory, when they previously perceived eggs deposited by herbivores insect which reliably indicates that larvae will soon hatch and feed on the plants. Using Arabidopsis thaliana and Pieris brassicae we addressed the question whether prior egg deposition affects transcriptional changes in feeding-damaged plants. We determined the transcriptomes of Arabidopsis leaves that (i) were for six days deposited with eggs and suffered larval feeding for two days, (ii) suffered larval feeding for two days without prior egg deposition or (iii) were left untreated.

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Transcription profiling of Arabidopsis coi1-1 gl1 plants challenged for 3-5 hr with Pieris rapae larvae

Project description:Arabidopsis coi1-1 gl1 plants were challenged for 3-5 hr with Pieris rapae larvae

2004-11-01 | E-MEXP-160 | biostudies-arrayexpress