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The transcriptional regulator LysG of Mycobacterium tuberculosis activates lysE in a lysine-dependent manner

ABSTRACT: The transcriptional regulator LysG of Mycobacterium tuberculosis activates lysE in a lysine-dependent manner

PROVIDER: PRJEB65014 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2103720_1.fastq.gz	Fastqsanger.gz
	ERR2103720_2.fastq.gz	Fastqsanger.gz
	ERR2103721_1.fastq.gz	Fastqsanger.gz
	ERR2103721_2.fastq.gz	Fastqsanger.gz
	ERR2103722_1.fastq.gz	Fastqsanger.gz

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Mycobacterium tuberculosis

Project description:The transcriptional regulator LysG of Mycobacterium tuberculosis activates lysE in a lysine-dependent manner

| PRJNA379276 | ENA

The transcriptional regulator LysG of Mycobacterium tuberculosis activates lysE in a lysine-dependent manner

Project description:To evaluate what genes are under the transcriptional control of LysG(Mt), a whole genome differential gene expression analysis of Wt Mt (Wt_Mtb) and del_ysG(1985c) was done, based on RNA-seq. Both were grown in a minimal medium supplemented with either 5 mM lysine, 10 mM ammonium or 3.34 mM histidine as sole nitrogen source. TruSeq Stranded mRNA Library Prep Kit from (Illumina, (San Diego, CA, USA) was used to prepare the cDNA libraries to analyze the whole transcriptome. An additional library was prepared from a pool of all isolated RNA according to the protocol of Pfeifer-Sancar et al. focusing on native 5'-ends of transcripts.

2017-09-03 | E-MTAB-6011 | biostudies-arrayexpress

MntR(Rv2788): a transcriptional regulator that controls manganese homeostasis in Mycobacterium tuberculosis.

2015-12-08 | GSE70812 | GEO

MntR(Rv2788): a transcriptional regulator that controls manganese homeostasis in Mycobacterium tuberculosis.

Project description:Transcriptional profiling of SirR and manganese regulated expression of genes in Mycobacterium tuberculosis strains comparing high manganese vs. low manganese in Rv (wild type Mycobacterium tuberculosis) and ST70 (mntR mutant strain of Mycobacterium tuberculosis) Two strains each with two conditions experiment, Rv (Mycobacterium tuberculosis wild type strain) high manganese vs. low manganese and ST70 (mntR mutant strain of Mycobacterium tuberculosis) high manganese vs. low manganese. Number of biological replicates is 3 for each condition for each strain.

2015-12-08 | E-GEOD-70812 | biostudies-arrayexpress

The transcriptional regulator LysG (Rv1985c) of Mycobacterium tuberculosis activates lysE (Rv1986) in a lysine-dependent manner.

Project description:The Mycobacterium tuberculosis protein encoded by the Rv1986 gene is a target for memory T cells in patients with tuberculosis, and shows strong similarities to a lysine exporter LysE of Corynebacterium glutamicum. During infection, the pathogen Mycobacterium tuberculosis adapts its metabolism to environmental changes. In this study, we found that the expression of Rv1986 is controlled by Rv1985c. Rv1985c is located directly upstream of Rv1986 with an overlapping promoter region between both genes. Semiquantitative reverse transcription PCR using an isogenic mutant of Mycobacterium tuberculosis lacking Rv1985c showed that in the presence of lysine, Rv1985c protein positively upregulated the expression of Rv1986. RNA sequencing revealed the transcription start points for both transcripts and overlapping promoters. An inverted repeat in the center of the intergenic region was identified, and binding of Rv1985c protein to the intergenic region was confirmed by electrophoretic mobility shift assays. Whole transcriptome expression analysis and RNAsequencing showed downregulated transcription of ppsBCD in the Rv1985c-mutant compared to the wild type strain. Taken together, our findings characterize the regulatory network of Rv1985c in Mycobacterium tuberculosis. Due to their similarity of an orthologous gene pair in Corynebacterium glutamicum, we suggest to rename Rv1985c to lysG(Mt), and Rv1986 to lysE(Mt).

| S-EPMC5648196 | biostudies-literature

Deficiency for SAMHD1 activates MDA5 in a cGAS/STING-dependent manner

Project description:Deficiency for SAMHD1 activates MDA5 in a cGAS/STING-dependent manner

| PRJNA869555 | ENA

Lineage-specific super-enhancer activates genes in a promoter-selective manner

Project description:Lineage-specific super-enhancer activates genes in a promoter-selective manner

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Deficiency for SAMHD1 activates MDA5 in a cGAS/STING-dependent manner II

Project description:Deficiency for SAMHD1 activates MDA5 in a cGAS/STING-dependent manner II

| PRJNA886727 | ENA

Deficiency for SAMHD1 activates MDA5 in a cGAS/STING-dependent manner [2]

Project description:Deficiency for SAMHD1 activates MDA5 in a cGAS/STING-dependent manner [2]