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DdSalPurp

ABSTRACT: Salix purpurea

PROVIDER: PRJEB65684 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	ERR12015742.fastq.gz	Fastqsanger.gz
	ERR12035266_1.fastq.gz	Fastqsanger.gz
	ERR12035266_2.fastq.gz	Fastqsanger.gz
	ERR12245591_1.fastq.gz	Fastqsanger.gz
	ERR12245591_2.fastq.gz	Fastqsanger.gz

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Transcriptome analysis of contrasting resistance to herbivory by empoasca fabae in two shrub willow species and their hybrid progeny

Project description:Shrub willow (Salix spp.), a short rotation woody biomass crop, has superior properties as a perennial energy crop for the Northeast and Midwest US. However, the insect pest potato leafhopper Empoasca fabae (Harris) (PLH) can cause serious damage and reduce yield of susceptible genotypes. Currently, the willow cultivars in use display varying levels of susceptibility under PLH infestation. However, genes and markers for resistance to PLH are not yet available for marker-assisted selection in breeding. In this study, transcriptome differences between a resistant genotype 94006 (S. purpurea) and a susceptible cultivar ‘Jorr’ (S. viminalis), and their hybrid progeny were determined. Over 600 million RNA-Seq reads were generated and mapped to the Salix purpurea reference transcriptome. Gene expression analyses revealed the unique defense mechanism in resistant genotype 94006 that involves PLH-induced secondary cell wall modification. In the susceptible genotypes, genes involved in programed cell death were highly expressed, explaining the necrosis symptoms after potato leafhopper feeding. Overall, the discovery of resistance genes and defense mechanisms provides new resources for shrub willow breeding and research in the future.

2020-01-15 | GSE143616 | GEO

Salix purpurea transcriptome

Project description:Salix purpurea transcriptome

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Project description:Salix purpurea overview

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Salix purpurea cultivar:94001

Project description:Salix purpurea 94001 Assembly

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Project description:Salix purpurea genome sequencing

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Salix purpurea cultivar:94003

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Salix purpurea cultivar:94003

Project description:Salix purpurea cultivar:94003 Assembly

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Project description:Monoecious Salix purpurea PacBio sequencing

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Functional analysis of Salix purpurea genes support roles for ARR17 and GATA15 as master regulators of sex determination

Project description:The Salicaceae family is of growing interest in the study of dioecy in plants because the sex determination region (SDR) has been shown to be highly dynamic, with differing locations and heterogametic systems across taxa. Previous studies investigating the mechanisms regulating sex in the genus Salix have been limited to genome resequencing and differential expression, which are mostly descriptive in nature, and functional validation of candidate sex determination genes has not been conducted. Here we use functional analysis to test a suite of previously identified candidate genes involved in sex determination and sex dimorphism in the bioenergy shrub willow Salix purpurea. Six candidate master regulator genes for sex determination were overexpressed in Arabidopsis, followed by floral proteome analysis. Eleven transcription factors with predicted roles in mediating sex dimorphism downstream of the SDR were tested using DAP-Seq in both male and female S. purpurea DNA. The results of this study provide further evidence to support models for the roles of ARR17 and GATA15 as master regulator genes of sex determination in S. purpurea, contributing to a regulatory system that is notably different from that of the related genus Populus. Two transcription factors, an AP2/ERF family gene and a homeodomain-like transcription factor, have evidence supporting roles in downstream regulation of sex dimorphism.

2023-02-01 | MSV000091180 | MassIVE

ddSalPurp

Project description:Salix purpurea, genomic and transcriptomic data

| PRJEB65683 | ENA