Project description:We used PacBio data to identify more reliable transcripts from hESC, based on which we can estimate gene/transcript abundance better from Illumina data. PacBio long reads and Illumina short reads were generated from the same hESC cell line H1. PacBio reads were error-corrected by Illumina reads to identify transcripts. rSeq is used to estimate gene/transcript abundance of the identified transcriptome.

2013-10-30 | E-GEOD-51861 | biostudies-arrayexpress

RNA-seq (Illumina and PacBio) of hESC

Project description:We used PacBio data to identify more reliable transcripts from hESC, based on which we can estimate gene/transcript abundance better from Illumina data.

2013-10-30 | GSE51861 | GEO

Ilex asprella var. asprella

Project description:Ilex asprella var. asprella Transcriptome or Gene expression

| PRJNA236423 | ENA

Decoding drought tolerance mechanisms in Holm oak (Quercus ilex) throught a combined transcriptomics and proteomics analysis

Project description:Transcript profiling of leaves from Quercus ilex seedlings subjected to well-watering and drought-stress (irrigation withdrawal) conditions

2020-04-03 | GSE145009 | GEO

Shotgun sequencing for microsatellite identification in Ilex paraguariensis (Aquifoliaceae).

Project description:UNLABELLED: PREMISE OF THE STUDY:Ilex paraguariensis is a native tree species from Brazil, Argentina, and Paraguay that is used in the production of beverages, medicines, and cosmetics. Primers flanking microsatellites were developed to investigate genetic parameters in the species. • METHODS AND RESULTS:Using microsatellites cloned from an I. paraguariensis shotgun genomic library, 25 pairs of primers were designed and synthesized. Levels of polymorphism were evaluated in 24 individuals from two populations. Twenty loci were polymorphic, and an average of 4.8 and 4.5 alleles per locus were detected in the two populations, respectively. The mean observed heterozygosity was lower than the expected heterozygosity (0.54 vs. 0.60), indicating a departure from Hardy-Weinberg equilibrium and suggesting endogamy in both populations. • CONCLUSIONS:The reported set of markers is highly informative and constitutes a powerful tool for the development of genetic characterization studies in I. paraguariensis.

| S-EPMC4105281 | biostudies-literature

Ilex cornuta

Project description:Ilex cornuta Transcriptome

| PRJNA399054 | ENA