Project description:Adult female three-spined stickleback fish (Gasterosteus aculeatus) were exposed to 10 individual chemicals, 26 mixtures of these chemicals, or control conditions in a flow-through system for 4 days. Transcriptomics was performed on liver samples by microarray. The main aims were to determine molecular signatures induced by these chemicals in the three-spined stickleback, discover whether these persisted in chemical mixtures and identify non-additive molecular responses in chemical mixtures exposures.
Project description:The data comes from a study, where three-spined sticklebacks (Gasterosteus aculeatus) were exposed to di-n-butyl phthalate (DBP) and 17α ethinyl-oestradiol (EE2) at nominal concentrations 35 μg/L and 40 ng/L, respectively, for four days. The aim of the study was to obtain insight into the acute transcriptional responses putatively associated with endocrine disruption. RNA samples from the testes of eight individuals fish per treatment (including solvent controls, exposed only to DMSO) were used in the microarray analysis, covering the expression of approximately 21000 genes.
Project description:samples from mouse Skeletal Muscle, analysis different expression profiling<br>HDAC4：Sciatic nerve transection model was prepared after injection of HDAC4-shRNA lentivirus into the tibialis anterior muscle of mice. 14 days later, tibialis anterior muscle was obtained. DenSciatic nerve transection model was prepared after injection of empty vector virus into the tibialis anterior muscle of mice. 14 days later, tibialis anterior muscle was obtained. N: The empty vector virus was injected into muscles from the sham group. 14 days later, tibialis anterior muscle was obtained.
