Project description:Geographical location influences the composition of the gut microbiota in wild house mice (Mus musculus domesticus) at a fine spatial scale
Project description:We employed RNA-sequencing to identify differentially expressed genes between two wild-derived inbred mouse strains. These strains exhibit high collateral blood vessel densities, but differ in widely in infarct volume following middle artery occlusion. This experiment helped facilitate identification of candidate loci underlying this differential response.
Project description:Accurate and reproducible quantitation of target genes depends on correct normalization. Historically, genes with variable tissue transcription e.g. GAPDH, have been used as normalization factors which is problematic, particularly in clinical samples which often are derived from different tissue sources. Using a large-scale gene database (GeneChip (Affymetrix U133A) dataset of 36 gastrointestinal tumors and normal tissues), we identified 8 candidate reference genes that were highly expressed with low variability and established expression levels by real-time RT-PCR in an independent set of GI tissue samples (n=42). Keywords: house-keeping, intestine, carcinoid