Project description:Four spider mites strains, 1-3 day old adult females with and without Wolbachia infection (FI, FU),and 1 day old adult males with and without Wolbachia infection (MI, MU).The four samples were sequenced on the Illumina HiSeq2000 platform.
Project description:Four spider mites strains, 1-3 day old adult females with and without Wolbachia infection (Turt_FI, Turt_Fu),and 1 day old adult males with and without Wolbachia infection (Turt_MI, Turt_MU).The four samples were sequenced on the Illumina HiSeq2000 platform.
Project description:Laodelphax striatellus is naturally infected with the Wolbachia strain wStri, which significantly increase the fecundity of its host. Wolbachia-infected females produce 30%–40% more eggs than Wolbachia-uninfected females. MicroRNAs (miRNAs) are a class of endogenous non-coding small RNAs that play critical roles in the regulation of gene expression at post-transcriptional level. Here we report the differentially expressed miRNAs between Wolbachia-infected and Wolbachia-uninfected strains of L. striatellus ovaries. Our data may be helpful to explore the molecular mechanisms by which Wolbachia increase the fecundity of Laodelphax striatellus.
Project description:We sequenced messenger RNA from mixed stages of the two-spotted spider mite (Tetranychus urticae) reared on bean (Phaseolus vulgaris cv California Red Kidney; the laboratory host plant for mites) and two Arabidopsis thaliana accessions which were considered to either be susceptible (Kondara) or resistant (Bla-2) to mite feeding. This pilot experiment was conducted to assess gene expression differences of mites grown on sensitive versus resistant Arabidopsis accessions, as well as differences in mites feeding on different host species. The expression data was used for gene model validation of genes predicted by EuGene in the spider mite genome and to assess gene expression levels. Examination of gene expression of spider mites reared on beans and two Arabidopsis accessions (Kondara and Bla-2).
Project description:We sequenced messenger RNA from mixed stages of the two-spotted spider mite (Tetranychus urticae) reared on bean (Phaseolus vulgaris cv California Red Kidney; the laboratory host plant for mites) and two Arabidopsis thaliana accessions which were considered to either be susceptible (Kondara) or resistant (Bla-2) to mite feeding. This pilot experiment was conducted to assess gene expression differences of mites grown on sensitive versus resistant Arabidopsis accessions, as well as differences in mites feeding on different host species. The expression data was used for gene model validation of genes predicted by EuGene in the spider mite genome and to assess gene expression levels.
Project description:Wolbachia, an endosymbiotic bacterium, is being investigated as a vector control agent in several insect species. Along with the well known classical reproductive parasitism Wolbachia employs against its host to spread within the population, it is emerging that the bacteria can protect the host against pathogens and reduced pathogen transmission. Anopheles mosquitoes, which transmit malaria, have never been found to harbour Wolbachia in nature, and despite numerous transinfection attempts, no stable line has been developed. However recently, two strains of Wolbachia, wAlbB from Aedes albopictus, and wRi from Drosophila simulans were cultured in Anopheles gambiae Sua5B cells. These cell lines provides an amenable system to study Wolbachia-Anopheles interaction in the absence of a stable transinfected line. It has been proposed that the compromised vector competence of Wolbachia infected insects is due to an up regulation of the basal immune state. We therefore completed a genome wide expression profile of Wolbachia infected Anopheles, assessing both wAlbB and wRi infected cells in parallel against uninfected Sua5B cells.
Project description:Transcriptional profiling of Drosophila melanogaster larval testes with and without the wMel strain of Wolbachia and found that 296 genes had at least a 1.5 fold change [q-value (%)<5%] in transcript levels, with 167 genes up-regulated and 129 genes down-regulated when comparing Wolbachia-infected flies to uninfected ones. Differential expression of genes related to metabolism, immunity, reproduction and other functions were observed.
Project description:Transcriptional profiling of Drosophila melanogaster larval testes with and without the wMel strain of Wolbachia and found that 296 genes had at least a 1.5 fold change [q-value (%)<5%] in transcript levels, with 167 genes up-regulated and 129 genes down-regulated when comparing Wolbachia-infected flies to uninfected ones. Differential expression of genes related to metabolism, immunity, reproduction and other functions were observed. Two-condition experiment, Wolbachia-infected vs. Wolbachia-uninfected testes. Biological replicates: 3 control, 3 infected, independently grown and dissected. One replicate per array.