Dataset Information

Presence of hypervirulence-associated determinants in Klebsiella pneumoniae from hospitalized patients in Germany

ABSTRACT: A collection of 67 potentially hypervirulent K. pneumoniae isolates was compiled. Genomic DNA was isolated and whole genome sequencing (WGS) was performed using Illumina® MiSeq, Illumina® NextSeq and MinIon, single and combined methods. Phylogenetic and genetic markers, such as capsule types and multi-locus sequence types, and virulence factors were extracted from NGS data.

PROVIDER: PRJEB67454 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Similar Datasets

Project description:The emergence of hypervirulent Klebsiella pneumoniae (hvKP) strains poses a significant threat to public health due to their high mortality rates and propensity to cause severe community-acquired infections in otherwise healthy individuals. The ability of hvKP to form biofilms and produce a protective capsule contributes to its enhanced virulence and is a significant challenge to effective antibiotic treatment. Therefore, understanding the molecular mechanisms underlying hvKP virulence and biofilm formation is crucial for developing new therapeutic strategies. Polyphosphate Kinase 1 (PPK1) is an enzyme responsible for inorganic polyphosphate synthesis and plays a vital role in regulating various physiological processes in bacteria. In this study, we investigated the impact of polyP metabolism on the biofilm and capsule formation and virulence traits in hvKP using Dictyostelium discoideum amoeba as a model host. We found that the PPK1 null-mutant was impaired in biofilm and capsule formation and showed attenuated virulence in D. discoideum compared to the wild-type strain. We performed a shotgun proteomic analysis of the PPK1 mutant and wild-type strain to gain further insight into the underlying molecular mechanism. The results revealed that the PPK1 mutant had a differential expression of proteins (DEP) involved in capsule synthesis (Wzi - Ugd), biofilm formation (MrkC-D-H), synthesis of the colibactin genotoxin precursor (ClbB), as well as proteins associated with the synthesis and modification of lipid A (ArnB -LpxC - PagP). These proteomic findings corroborate the phenotypic observations and indicate that the PPK1 mutation is associated with impaired biofilm and capsule formation and attenuated virulence in hypervirulent K. pneumoniae. Overall, our study highlights the importance of polyP synthesis in regulating extracellular biomolecules and virulence in K. pneumoniae and provides insights into potential therapeutic targets for treating K. pneumoniae infections.

Dataset Information

Presence of hypervirulence-associated determinants in Klebsiella pneumoniae from hospitalized patients in Germany

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets