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Genome sequencing of Bifidobacterium pseudocatenulatum CECT 7765.

ABSTRACT: Bifidobacterium pseudocatenulatum CECT 7765 genome was sequenced by using MiSeq Illumina Technology.

PROVIDER: PRJEB6926 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	ERR593545_1.fastq.gz	Fastqsanger.gz
	ERR593545_2.fastq.gz	Fastqsanger.gz

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Comparative transcriptomic profiling of liver tissue from wild-type C57BL-6 mice fed a high-fat diet (HFD) supplemented with the probiotic (P) Bifidobacterium pseudocatenulatum CECT 7765.

2012-11-20 | GSE40638 | GEO

RNA sequencing of Bifidobacterium pseudocatenulatum MP80 grown on Lactose 2'-FL and LNFP-1

Project description:RNA sequencing revolutionized the bacterial gene expression analysis. The objective of this study was to identify the genes involved in metabolism of 2'-FL and LNFP1 in Bifidobacterium pseudocatenulatum MP80. We have obtained a list of genes upregulated in Bifidobacterium pseudocatenulatum MP80 when it is grown in 2% 2'-FL and LNFP1. Lactose grown samples were used as the control.

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Comparative transcriptomic profiling of liver tissue from wild-type C57BL-6 mice fed a high-fat diet (HFD) supplemented with the probiotic (P) Bifidobacterium pseudocatenulatum CECT 7765.

Project description:We used Affymetrix microarrays to investigate gene expression changes in the liver of wild-type C57BL-6 mice exposed to a high-fat diet that might have been caused by the oral consumption of the probiotic B. pseudocatenulatum CECT 7765. The aim of this work was to determine whether the daily intake (by oral gavage) of the probiotic (P) B. pseudocatenulatum for seven weeks exerted any modulatory effects, at the level of gene expression, in the liver of C57BL-6 male mice exposed to a high-fat diet (HFD). Male mice were randomly assigned to four experimental groups (n= 5 animals per group) as follows: (1) control group, fed a standard diet (SD); (2) obese group, fed a high-fat diet (HFD); (3) a group that received the SD and a daily dose of the probiotic (1M-CM-^W109 CFU B. pseudocatenulatum CECT 7765) (SD+P); and (d) an obese group that was fed the HFD and a daily dose of the probiotic (1M-CM-^W109 CFU B. pseudocatenulatum CECT 7765) (HFD+P). At the end of the experimental procedure total RNA was extracted from the liver to compare differential gene expression between the groups. Liver differential gene expression after 7 weeks of supplementation between: 1) the HFD group and the SD group (effects of the high-fat diet); 2) the HFD+P and the HFD (effects of the probiotic on the consumption of a high-fat diet) and 3) the SD+P group and the SD (direct effects of the probiotic on the liver of animals consuming a normal diet).

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