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Temporal variation of lagoon-associated and pelagic (open ocean) planktonic 16S rRNA-gene sequences (Mo’orea, French Polynesia).

ABSTRACT: Planktonic 16S rRNA gene sequences (V4/V5 region) from water samples collected inside the northern coral-reef lagoon of Mo’orea (French Polynesia) and 4 km offshore, in the tropical open ocean.

PROVIDER: PRJEB69514 | ENA |

REPOSITORIES: ENA

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Temporal variation of lagoon-associated and pelagic (open ocean) planktonic 18S rRNA-gene sequences (Mo’orea, French Polynesia).

Project description:Planktonic 18S rRNA gene sequences (V4 region) from water samples collected inside the northern coral-reef lagoon of Mo’orea (French Polynesia) and 4 km offshore, in the tropical open ocean.

| PRJEB69515 | ENA

Gene expression profiling of native mussels from Venice lagoon area

Project description:We examined gene expression profiling of native mussels that were sampled in early summer 2003 from sites of the Venice lagoon area known to be differently affected by chemical pollution: Sites 1 and 2 close to the industrial district of Marghera and Site 3 close to the Lido lagoon outlet. Site 4, a current mussel farm located offshore, has been chosen as source of reference targets for microarray hybridizations. We have limited the preliminary assessment to the digestive gland. Digestive gland total RNA of each Site was hybridized in competition with the offshore mussels (Site 4 - Reference) and the relative abundance of each gene was measured by directly comparing fluorescent signals for each probe. We carried out two separate hybridizations for each site of the Venice lagoon area.. Keywords = digestive gland Keywords = Venice lagoon Keywords = chemical pollution Keywords = native mussels Keywords = transcriptional profiling Keywords: ordered

2006-10-10 | GSE2184 | GEO

Coral-associated planktonic 16S rRNA-gene sequences from an Acropora pulchra colony

Project description:Planktonic 16S rRNA gene sequences (V4/V5 region) from water samples collected around an Acropora pulchra colony (Tema’e Beach reef, Mo’orea)

| PRJEB54595 | ENA

Diuron tolerance and potential degradation by pelagic microbiomes in the Great Barrier Reef lagoon.

Project description:Diuron is a herbicide commonly used in agricultural areas where excess application causes it to leach into rivers, reach sensitive marine environments like the Great Barrier Reef (GBR) lagoon and pose risks to marine life. To investigate the impact of diuron on whole prokaryotic communities that underpin the marine food web and are integral to coral reef health, GBR lagoon water was incubated with diuron at environmentally-relevant concentration (8 µg/L), and sequenced at specific time points over the following year. 16S rRNA gene amplicon profiling revealed no significant short- or long-term effect of diuron on microbiome structure. The relative abundance of prokaryotic phototrophs was not significantly altered by diuron, which suggests that they were largely tolerant at this concentration. Assembly of a metagenome derived from waters sampled at a similar location in the GBR lagoon did not reveal the presence of mutations in the cyanobacterial photosystem that could explain diuron tolerance. However, resident phages displayed several variants of this gene and could potentially play a role in tolerance acquisition. Slow biodegradation of diuron was reported in the incubation flasks, but no correlation with the relative abundance of heterotrophs was evident. Analysis of metagenomic reads supports the hypothesis that previously uncharacterized hydrolases carried by low-abundance species may mediate herbicide degradation in the GBR lagoon. Overall, this study offers evidence that pelagic phototrophs of the GBR lagoon may be more tolerant of diuron than other tropical organisms, and that heterotrophs in the microbial seed bank may have the potential to degrade diuron and alleviate local anthropogenic stresses to inshore GBR ecosystems.

| S-EPMC4793316 | biostudies-literature

Experimental and Environmental Metabolomics of Coral Reef DOM from Moorea, French Polynesia (2019)

Project description:Non-targeted LC-MS/MS of PPL extracts from experimental and environmental seawater samples from coral reefs from Mo'orea (French Polynesia), collected in May 2019.

2021-08-25 | MSV000088021 | MassIVE

Octadecabacter arcticus 238

Project description:A psychrophilic marine microorganism collected 350 km offshore Deadhorse, Alaska.

| PRJNA19331 | ENA

French Polynesia Sponge Biodiversity

Project description:Poriferans Rift Apart: Molecular sponge biodiversity in Central and French Polynesia and comparison with adjacent marine provinces of the Central Indo-Pacific

| PRJEB54662 | ENA

New sponges from French Polynesia

Project description:New genus and species of lithistid demosponges from submarine caves in Nuku Hiva (Marquesas Islands) and Tahiti Iti (Society Islands), French Polynesia

| PRJEB45066 | ENA

Pelagic

Project description:Pelagic Metagenome

| PRJNA473552 | ENA

Understanding differential heat tolerance of the threatened mountainous star coral Orbicella faveolata from inshore and offshore reef sites in the Florida Keys using gene network analysis

Project description:We subjected three inshore and four offshore genotypes of the coral Orbicella faveolata to 30, 31, 32, or 33ºC for 31 days and measured photochemical efficiency (Fv/Fm), the types and relative abundance of dinoflagellate endosymbionts, and gene expression of the host and symbiont. All inshore coral genotypes, regardless of symbiont type, were significantly more thermotolerant than offshore genotypes based on declines in Fv/Fm. The most heat-tolerant inshore genotype (In1) was dominated by Durusdinium trenchii; all other genotypes were Breviolum-dominated, suggesting local adaptation or acclimatization contributes to the heat tolerance of inshore genotypes. After 31 days of heat stress, all coral genotypes (except In2) had lost most of their Breviolum and became dominated by D. trenchii. Host genotype In1 presented unique expression patterns of genes involved in heat shock response, immunity, and protein degradation. There were few changes in the symbiont transcriptomes of inshore corals under heat stress, but significant changes in symbiont gene expression from the offshore colonies, including increases in ribosomal and photosynthetic proteins. These data show that the differential thermotolerance between inshore and offshore O. faveolata in the Florida Keys is associated with statistically significant differences in both host and symbiont gene expression that provide insights into the mechanisms underlying holobiont heat tolerance.

2024-02-06 | GSE225085 | GEO

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