Project description:Lysinibacillus varians GY32 was isolated from river sediment of electronic waste recycling site. Its invariably filament-to-rod cell cycle represents a novel bacteria morphogenesis that is crucial in understanding cell division coordination with lifecycle and environmental bacteria adaptation. A description of genes and biological processes involved in the special filament-to-rod cell cycle of L. varians GY32 is within reach.
Project description:The hypothalamic suprachiasmatic (SCN) clock contains several neurochemically defined cell groups that contribute to the genesis of circadian rhythms. Using cell specific and genetically-targeted approaches we have confirmed an indispensable role for vasoactive intestinal polypeptide expressing SCN (SCNVIP) neurons in generating the mammalian locomotor activity (LMA) circadian rhythm. Optogenetic-assisted circuit mapping revealed functional, di-synaptic connectivity between SCNVIP neurons and dorsomedial hypothalamic neurons, providing a circuit substrate by which SCNVIP neurons may regulate LMA rhythms. In vivo photometry revealed that while SCNVIP neurons are acutely responsive to light, their activity is otherwise behavioral state invariant. Single-nuclei RNA-sequencing revealed SCNVIP neurons comprise two transcriptionally distinct subtypes, including putative pacemaker and non-pacemaker populations. Given that SCNVIP neurons constitute ~10% of the total SCN population, and that other cell groups were unable to sustain coherent circadian LMA rhythms following SCNVIP disruption, our findings demonstrate a disproportionately large influence of the SCNVIP cell population on pacemaker function.
Project description:Lysinibacillus varians GY32 is a filamentous bacteria that can generate electricity in microbial fuel cells. To find potential genes participating in the electron transfer to electrode of Lysinibacillus varians GY32, we compared the gene expression profiles of this bacteria with yeast extract as electron donor and two electron acceptors, i.e. oxygen and electrode in microbial fuel cells. The results showed that several cytochrome c genes might play specific roles in the extracellular electron transfer to electrode in this strain.
Project description:Lysinibacillus varians GY32 is a filamentous bacteria that can generate electricity in microbial fuel cells. To find potential genes participating in the electron transfer to electrode of Lysinibacillus varians GY32, we compared the gene expression profiles of this bacteria with acetate as electron donor and two electron acceptors, i.e. oxygen and electrode in microbial fuel cells. The results showed that several cytochrome c genes might play specific roles in the extracellular electron transfer to electrode in this strain.
