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RNA-Seq of influenza A/Puerto Rico/8/1934 (H1N1) virus infected monoclonal cell lines with depressed IFITM3 gene activity

ABSTRACT: RNA-Seq of influenza A/Puerto Rico/8/1934 (H1N1) virus infected monoclonal cell lines with depressed IFITM3 gene activity

PROVIDER: PRJEB70764 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR12329533_1.fastq.gz	Fastqsanger.gz
	ERR12329533_2.fastq.gz	Fastqsanger.gz
	ERR12329534_1.fastq.gz	Fastqsanger.gz
	ERR12329534_2.fastq.gz	Fastqsanger.gz
	ERR12329535_1.fastq.gz	Fastqsanger.gz

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RNA-Seq of influenza A/Puerto Rico/8/1934 (H1N1) virus infected monoclonal cell lines with depressed IFITM3 gene activity

Project description:In this study monoclonal cell lines carrying mutations in IFITM3 gene were obtained based on WI-38 VA13 cells. To research the involvement of the IFITM3 gene in cellular response to Influenza A virus infection, original WI-38 VA13 cells and the clones with depressed IFITM3 gene activity (F3, F5, Е12) were infected with influenza A/Puerto Rico/8/1934 (H1N1) virus. RNA-seq analysis of infected cell lines after 24 h was performed on an Illumina NextSeq 500 platform. The same mock-infected cells were used as controls (0 hpi). PolyA RNA-enriched fraction was used for constructing of cDNA libraries. Differential expressed genes were identified using R package DESeq2.

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