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IlScoChen

ABSTRACT: Scotopteryx chenopodiata (shaded broad-bar)

PROVIDER: PRJEB71304 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	ERR12370429.fastq.gz	Fastqsanger.gz
	ERR13702756_1.fastq.gz	Fastqsanger.gz
	ERR13702756_2.fastq.gz	Fastqsanger.gz
	ERR13999094_1.fastq.gz	Fastqsanger.gz

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ilScoChen

Project description:Scotopteryx chenopodiata (shaded broad-bar), genomic and transcriptomic data

| PRJEB71303 | ENA

Nonspecific activities of the major herbicide-resistance gene BAR

Project description:Bialaphos resistance (BAR) and phosphinothricin acetyltransferase (PAT) genes, which convey resistance to the broad-spectrum herbicide phosphinothricin (also known as glufosinate) via N-acetylation, have been globally used in basic plant research and genetically engineered crops. Although early in vitro enzyme assays showed that recombinant BAR and PAT exhibit substrate preference toward phosphinothricin over the 20 proteinogenic amino acids, indirect effects of BAR-containing transgenes in planta, including modified amino acid levels, have been seen but without the identification of their direct causes. Combining metabolomics, plant genetics, and biochemical approaches, we show that transgenic BAR indeed converts two plant endogenous amino acids, aminoadipate and tryptophan, to their respective N-acetylated products in several plant species examined. We report the crystal structures of BAR, and further delineate structural basis for its substrate selectivity and catalytic mechanism. Through structure-guided protein engineering, we generated several BAR variants that display significantly reduced nonspecific activities compared to its wild-type counterpart in vivo. Our results demonstrate that transgenic expression of enzymes can result in unintended off-target metabolism arising from enzyme promiscuity. Understanding of such phenomena at the mechanistic level can facilitate the design of maximally insulated systems featuring heterologously expressed enzymes.

2017-12-05 | MTBLS553 | MetaboLights

ilScoBipu

Project description:Scotopteryx bipunctaria

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In Situ Proximity Labeling Identifies Lewy Pathology Molecular Interactions in the Human Brain

Project description:The intracellular misfolding and accumulation of alpha-synuclein into structures collectively called LP is a central phenomenon for the pathogenesis of synucleinopathies including Parkinson’s disease (PD) and Dementia with Lewy Bodies (DLB). Understanding the molecular architecture of LP is crucial for understanding synucleinopathy disease origins and progression. Here we used a technique called biotinylation by antibody recognition (BAR) to label total (BAR-SYN1) and pathological alpha-synuclein (BAR-PSER129) in situ for subsequent mass spectrometry analysis. This technique has broad potential to help understand the phenomenon of LP in primary human tissue and animal models.

2022-02-22 | PXD028830 | Pride

Scotopteryx bipunctaria

Project description:Scotopteryx bipunctaria overview

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Scotopteryx chenopodiata

Project description:Scotopteryx chenopodiata overview

| PRJNA1179576 | ENA

Whole-genome transcriptome analysis of tomato and Arabidopsis roots in the shade

Project description:RNA-seq analysis of arabidopsis and tomato roots in shaded and unshaded seedlings.

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ilEupSubu

Project description:Eupithecia subumbrata (shaded pug)

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Gene expression during dark-induced leaf abscission in Populus x tremula

Project description:Identification of abscission regulated genes Genes involved in auxin transport and response are down-regulated in axils of shaded leaves.

2015-05-28 | GSE69277 | GEO

ilScoBipu1

Project description:Scotopteryx bipunctaria genome assembly, ilScoBipu1

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