Ribosome profiling in C2C12 mouse proliferating myoblasts and differentiated myotubes.
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ABSTRACT: We monitored translation at nucleotide resolution in a genome-wide high throughput manner, using ribosome profiling on the murine C2C12 cell line, a model for skeletal muscle differentiation. We simplified the existing protocol and developed a data analysis pipeline to characterize translation initiation during differentiation of myoblasts into myotubes, to detect switches in the use of alternative translation initiation sites, as well as to quantify translation.
PROVIDER: PRJEB7207 | ENA |
REPOSITORIES: ENA
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