Project description:Advances in mass spectrometry (MS) instrumentation resulting in higher mass resolution and higher duty cycles have allowed deeper coverages of proteomes. The development of candidate RM 8461 Human Liver for Proteomics was undertaken to provide the community with a sample with suitable complexity for method development and validation, instrument and data validation, and determining identification QC thresholds. Herein we describe a comparison of methods and homogeneity assessment in which candidate RM 8461 Human Liver for Proteomics is solubilized, the proteins reduced, alkylated, digested and the tryptic peptides and their modifications are identified by bottom up LC/MS/MS to assess deep (e.g. thousands of identifications) protein coverage.
Project description:Western equine encephalitis virus (WEEV) is a mosquitoborne virus that reemerged in December 2023 in Argentina and Uruguay, causing a major outbreak. We investigated the outbreak using epidemiologic, entomological, and genomic analyses, focusing on WEEV circulation near the Argentina‒Uruguay border in Rio Grande do Sul state, Brazil. During November 2023‒April 2024, the outbreak in Argentina and Uruguay resulted in 217 human cases, 12 of which were fatal, and 2,548 equine cases. We determined cases on the basis of laboratory and clinical epidemiologic criteria. We characterized 3 fatal equine cases caused by a novel WEEV lineage identified through a nearly complete coding sequence analysis, which we propose as lineage C. Our findings highlight the importance of continued surveillance and equine vaccination to control future WEEV outbreaks in South America.
