Project description:To characterize the differentially expressed genes of miR340, we compared the gene expression profiles of miR-340 overexpressing human A549 cells with that of empty vector transfected A549 cells
Project description:CCAR1 mRNA have two transcripts, the 3.5 kb full length transcript and the 2.5 kb shorter transcript which contained a deletion in exon 15—exon 22 of full-length CCAR1 cDNA, where sequences mapped to 1196–3120 bp were missed. CCAR1L and CCAR1S shared a common N-terminus and a common C-terminal end, but the latter had a deletion of SAP domain. As a DNA/RNA-binding domain, the SAP motif is involved in chromosomal organization and apoptosis. To explaine the divergence of CCAR1L and CCAR1S in regulating apoptosis,we purified CCAR1L and CCAR1S from flag-CCAR1L or CCAR1S overexpressing A549 cells by performing IP-MS to screening the possible proteins which are specifically interacting with CCAR1L or CCAR1S.
Project description:Fresh peripheral blood mononuclear cells of four human donors were cultured together with either lung adenocarcinoma A549 cancer cells or A549-expressing H1N1 Sialidase cancer cells. These treatments induced the differentiation of donor cells into immunosuppressive MDSC-like cells, which were further subjected to bulk RNA sequencing. Computational analysis of RNA-Seq profiles of these cells was applied to understand the differences in their suppressive capacities.
Project description:The goals of this study are to compare NGS-derived whole transcriptome profiles (RNA-seq) of H5N1 infected A549 cells overexpressing either negative control mimic or miR-324-5p mimic
Project description:This experiment is designed to evaluate gene expression alteration following silencing SMAD2/3 and overexpressing CCT6A in A549 lung cancer cells. Total RNA were extraced from indicated stable cell lines treated with or with out TGF-β
