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Transcriptomic profile of valvular endothelial cells in normal and high glucose conditions, interacted with human monocytes (THP-1)

ABSTRACT: Transcriptomic profile of valvular endothelial cells in normal and high glucose conditions, interacted with human monocytes (THP-1)

PROVIDER: PRJEB72376 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR12642855_1.fastq.gz	Fastqsanger.gz
	ERR12642855_2.fastq.gz	Fastqsanger.gz
	ERR12642856_1.fastq.gz	Fastqsanger.gz
	ERR12642856_2.fastq.gz	Fastqsanger.gz
	ERR12642857_1.fastq.gz	Fastqsanger.gz

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Transcriptomic profile of valvular endothelial cells in normal and high glucose conditions, interacted with human monocytes (THP-1)

Project description:Human valvular endothelial cells were co-cultured with THP-1 monocytes in high glucose (33mM) or normal glucose (5mM) levels for 2 hours. Afterwards cells were lysed and total cellular RNA was extracted using TRIzol reagent.

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Glycoproteomes of naive and LPS stimulated human monocytes and THP-1 cells

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THP-1 human monocytes Transcriptome following exposure to S-nitrosoglutathione (GSNO)

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THP-1 human monocytes Transcriptome following exposure to S-nitrosoglutathione (GSNO)

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Differentially expressed genes in pneumococcus-infected THP-1 monocytes

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Expression data from THP-1 cells and THP-1 cells stimulated with PMA

Project description:Macrophages play a key role in both innate and adaptive immunity, but our knowledge on the changes in transcription regulation that occurs during their differentiation from monocytes is still limited. In this study, we used a meta-analysis followed by a systems biology approach for the identification of differentially expressed genes between monocytes and macrophages and possible regulators of these changes in transcription. Based on the pattern of gene expression change, transcription regulator analysis predicted a decrease in Enhancer of Zeste homolog 2 (EZH2), a histone 3 lysine 27 methyl transferase, activity after differentiation of monocytes into macrophages. This inhibition was validated by a significant decrease in trimethylated H3K27 during differentiation of both human primary monocytes into macrophages and the THP-1 cell line into macrophage-like cells. Overexpressing EZH2 during differentiation of monocytes and THP-1 cells obstructs cellular adhesion, thus preventing the first step in differentiation. Another facet of macrophage differentiation is the cessation of proliferation, and inhibition of EZH2 by the small molecule inhibitor GSK126 in THP-1 cells indeed impedes proliferation. This study shows an important part for epigenetic changes during monocyte differentiation. It highlights the role of EZH2 activity behind the changes needed in adhesion and proliferation mechanisms for macrophage formation. THP-1s were differentiated into macrophage like cells by PMA stimulation.

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