Project description:Cell states are regulated by extrinsic signals from various external factors such as intercellular interactions, and intrinsic gene expression. Although comprehensive cell state profiling has been attempted, it remains simultaneous analysis of signal activation has still been challenging. Multiplexed imaging is a technique acquiring multiple protein information at a single cell level as traditional immunofluorescence. However, the method often compromises resolution, hindering the analysis of intracellular localization dynamics and post-translational modifications of proteins. To address these limitations, we developed an erasable fluorescence method using disulfide linkers to label antibodies. We term these antibodies ‘Precise Emission Canceling Antibodies (PECAbs)’. PECAb allows for high-resolution iterative imaging with minimal non-specific binding. Automation enables our system to achieve reproducible quantitative analysis using 206 antibodies. The resulting quantitative data allow reconstruction of the spatiotemporal dynamics of signaling pathways over both long and short timescales. Additionally, combining this approach with sequential RNA-FISH can effectively classify cells and identify their signal activation states in human tissue. Overall, the PECAb system serves as a comprehensive platform for analyzing complex cell processes, from signal transduction to gene expression.

2024-04-21 | GSE242886 | GEO

Precise immuno-fluorescence canceling enables highly multiplexed imaging [scRNA-seq]

2024-04-21 | GSE242814 | GEO

Juvenile Fluorescence

Project description:Juvenile Fluorescence Raw sequence reads

| PRJNA436185 | ENA

Indocyanine green fluorescence imaging in liver metastasis from colorectal cancer

Project description:Interventions: A group:Traditional hepatotomy;B group:ICG fluorescence imaging hepatectomy Primary outcome(s): Number of liver lesions detected Study Design: Parallel

| 2699833 | ecrin-mdr-crc

Research for intraoperative navigation by ICG fluorescence imaging in laparoscopic liver resection

Project description:Interventions: intravenous administration of ICG Primary outcome(s): Success rate of the visualization of liver tumor by ICG fluorescence imaging. Study Design: Single arm Non-randomized

| 2639377 | ecrin-mdr-crc

The effect of 3D imaging device on the detection rate of adenoma in colonoscopy

Project description:Interventions: Gold Standard:Pathological examination;Index test:1. 3D imaging device colonoscopy; 2. 2D imaging device colonoscopy. Primary outcome(s): Adenoma detection rate;SEN, SPE, ACC, AUC of ROC Study Design: Sequential

| 2684296 | ecrin-mdr-crc

The multicenter research of 3D imaging device on the detection rate of adenoma in colonoscopy

| 2686006 | ecrin-mdr-crc