Project description:Whole genome microarrays were used to compare the transcriptional profile of Candida parapsilosis bcr1 knockout to wild type cells.
Project description:<p><em>Candida</em> species are the most common cause of opportunistic fungal infections. Rapid identification and novel approaches for the characterization of these fungi are of great interest to improve the diagnosis and the knowledge about their pathogenic properties. This study aimed to characterize clinical isolates of <em>Candida</em> spp. by proteomics (MALDI-TOF MS) and metabolomics (<sup>1</sup>H-NMR), and to correlate their metabolic profiles with <em>Candida</em> species, source of infection and different virulence associated parameters. In particular, 49 <em>Candida</em> strains from different sources (blood, n = 15; vagina, n = 18; respiratory tract, n = 16), belonging mainly to <em>C. albicans</em> complex (61%), <em>C. glabrata</em> (20%) and <em>C. parapsilosis</em> (12%) species were used. Several extracellular and intracellular metabolites showed significantly different concentrations among isolates recovered from different sources of infection, as well as among different <em>Candida</em> species. These metabolites were mainly related to the glycolysis or gluconeogenesis, tricarboxylic acid cycle, nucleic acid synthesis and amino acid and lipid metabolism. Moreover, we found specific metabolic fingerprints associated with the ability to form biofilm, the antifungal resistance (i.e. caspofungin and fluconazole) and the production of secreted aspartyl proteinase. In conclusion, <sup>1</sup>H-NMR-based metabolomics can be useful to deepen <em>Candida</em> spp. virulence and pathogenicity properties.</p>
Project description:This SuperSeries is composed of the following subset Series: GSE13717: Transcriptional profile of Candida parapsilosis in SD media GSE13722: Transcriptional response of Candida parapsilosis in low oxygen (hypoxic) conditions in SD media Refer to individual Series
Project description:Investigation of centromeres in the pathogenic yeast Candida parapsilosis, shows that the location of two centromeres are polymorphic within this species. The centromeres consist of large inverted repeats (IRs), surrounding unique sequences. New (neo) centromeres have emerged in one C. parapsilosis isolate even though the original CEN location is undamaged. The neocentromeres do not contain IRs, and have no obvious sequence features.
Project description:This SuperSeries is composed of the following subset Series: GSE32712: Transcriptional profile of Candida parapsilosis CLIB214 21% Oxygen (normoxia) versus at 1% oxygen (hypoxia). GSE32713: Transcriptional profile of Candida parapsilosis CLIB214 versus UPC2 delete, both at 1% oxygen (hypoxia) GSE32714: Transcriptional landscape of Candida parapsilosis Refer to individual Series