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TRAP-seq of astrocytes in the APP NL-F knock-in model of ß-amyloidopathy

ABSTRACT: TRAP-seq of astrocytes in the APP NL-F knock-in model of ß-amyloidopathy

PROVIDER: PRJEB74066 | ENA |

REPOSITORIES: ENA

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TRAP-seq of astrocytes in the APP NL-F knock-in model of ß-amyloidopathy

Project description:While mouse models of ß-amyloidopathy recapitulate aspects of Alzheimer's disease pathology, including plaque formation and synapse loss, most progress at a speed such that outcomes are studied in the mature mouse, rather than the aged mouse. Here we investigated Aß-dependent changes to astrocytes in a knock-in model of ß-amyloidopathy (APP NL-F) that is known to progress more slowly.

2024-03-21 | E-MTAB-13936 | biostudies-arrayexpress

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Project description:Single cell sequencing of astrocytes in the APP NL-F and APP PS1 models of ß-amyloidopathy

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RNA-seq and TRAP-seq of astrocytes and brain tissue in mouse models of Alzheimer's pathology

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A high-fat diet exacerbates the Alzheimer's disease pathology in the hippocampus of the App[NL-F/NL-F] knock-in mouse model

Project description:To investigate the effects of diabetic conditions on the development of AD pathology, we fed wild-type and Alzheimer's disease model (App[NL-F/NL-F]) mice with control or high fat diet from 6 months after birth for 12 months, then examined behavior, brain pathology and gene expression profiles. Both groups of mice fed with high fat diet exhibited diabetic conditions, and App[NL-F/NL-F] mice exhibited aggravated cognitive impairment with enhanced Alzheimer's disease pathology.

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Transcription pattern in Normal (NL) and Down's Syndrome (DS) Astrocytes

Project description:We performed a broad-spectrum microarray analysis using mRNA from 3 NL and 5 DS astrocyte cultures. The microarray output was validated for several genes To explore the relation between gene expression and potential oxidative stress in DS samples, we also evaluated the transcription profile in NL astrocytes subjected to mild oxidative stress (50µM H2O2 for 24 hr).

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