Project description:We report the application of Whole Genome Bisulfite Sequencing to identify differentially methylated regions (DMR) in two RNAi transgenic lines, with dowregulated expression of the demeter-like gene PtaDML10, in comparisson to wild-type plants of Populus tremula x Populus alba plants.
Project description:The Poplar transcriptome was analyzed in Populus tremulaxPopulus alba clone 717-1B4 control roots and in two poplar lines overexpressing MiSSP7. We performed 9 hybridizations (NimbleGen) with samples derived from Populus tremulaxPopulus alba clone 717-1B4 control roots, as well as from roots of LINE1 and LINE2 MiSSP7 overexpressor poplars (3 biological replicates each). All samples were labeled with Cy3.
