Project description:We used scRNA-seq to profile 75,218 single cell transcriptomes of Pristina leidyi, a highly regenerative and asexually-reproducing freshwater annelid. We characterized all major adult cell types, their transcription factors and gene networks, and uncovered an abundant population of putative stem cells with a pluripotent signature.
2023-04-28 | GSE230505 | GEO
Project description:RNA-seq of regenerating and non-regenerating Lumbriculus variegatus worms
Project description:The objective of this project was to investigate the impacts of oil exposure in combination with environmental stressors on gene expression in developing C. variegatus.
Project description:The objective of this project was to expose larval Cyprinodon variegatus to oil/hypoxia stressors for 48 hrs, then allow 48 hours of recovery and depuration.
Project description:The adhesion of flavescence dorée phytoplasma to the midgut epithelium cells of their insect vectors is partially mediated by the Variable Membrane Protein A (VmpA), an adhesin which shows lectin properties. In order to identify the insect receptor for VmpA, we lokked for Euscelidius variegatus cells proteins interacting with recombinant VmpA-His6 by mass spectrometry analysis of VmpA-E. variegatus protein complexes formed upon in vitro interaction assays.