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Single-nucleus RNA-Sequencing (sci-RNA-seq3) of whole livers from 48 Four Core Genotype (FCG) mice

ABSTRACT: Single-nucleus RNA-Sequencing (sci-RNA-seq3) of whole livers from 48 Four Core Genotype (FCG) mice

PROVIDER: PRJEB74491 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR12829300_1.fastq.gz	Fastqsanger.gz
	ERR12829300_2.fastq.gz	Fastqsanger.gz
	ERR12829301_1.fastq.gz	Fastqsanger.gz
	ERR12829301_2.fastq.gz	Fastqsanger.gz
	ERR12829302_1.fastq.gz	Fastqsanger.gz

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Single-nucleus RNA-Sequencing (sci-RNA-seq3) of whole livers from 48 Four Core Genotype (FCG) mice

Project description:This repository contains single-nucleus gene expression profiling from 48 mouse livers of the Four Core Genotypes cross. By crossing mice with both a deletion of the sex-determining factor Sry on the Y-chromosome and a transgenic insertion of Sry on Chromosome 3, four combinations of gonadal (testis or ovaries) and chromosomal (XX or XY) are generated, namely XYSry-Chr3Sry+ (gonadal and chromosomal males), XYSry- (gonadal females, chromosomal males), XX (gonadal and chromosomal females), XXChr3Sry+ (gonadal males, chromosomal females). The transgenes are on a C57BL6 genetic background. From all four genotypes, whole livers were dissected and flash frozen, followed by nuclear extraction and fixation for sci-RNA-seq3.

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Single-nucleus RNA-Sequencing of whole livers from Four Core Genotype (FCG) mice

Project description:This repository contains single-nucleus gene expression profiling from mouse livers of the Four Core Genotypes cross. By crossing mice with both a deletion of the sex-determining factor Sry on the Y-chromosome and a transgenic insertion of Sry on Chromosome 3, four combinations of gonadal (testis or ovaries) and chromosomal (XX or XY) are generated, namely XYSry-Chr3Sry+ (gonadal and chromosomal males), XYSry- (gonadal females, chromosomal males), XX (gonadal and chromosomal females), XXChr3Sry+ (gonadal males, chromosomal females). The transgenes are on a C57BL6 genetic background. From all four genotypes, whole livers were dissected and flash frozen, followed by nuclear extraction and fixation for sci-RNA-seq3.

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