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H99 derived uniconzole (0.25ug/mL and 2ug/mL) adaptors

ABSTRACT: H99 derived uniconzole (0.25ug/mL and 2ug/mL) adaptors

PROVIDER: PRJEB75221 | ENA |

REPOSITORIES: ENA

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H99 derived Uniconazole (1ug/mL) adaptors

Project description:H99 derived Uniconazole (1ug/mL) adaptors

| PRJEB75213 | ENA

H99 derived uniconzole (0.25ug/mL and 2ug/mL) adaptors

Project description:H99 derived 2 ug/mL uniconazole adaptors: Approximately 1 million cells of Crypotoccus neoformans lab strain H99 were spread on YPD-agar plate supplemented with 2 ug/mL uniconazole. After 5 days incubation of the plate at 30℃, randomly 30 adaptors were chosen. 21 adaptors were resistant and were sequenced. H99 derived 0.25 ug/mL uniconazole adaptors: Cells of H99 were adjusted to 2500 cells/mL. The culture was supplemented with 0.25 ug/mL uniconazole. After 72 h incubation, the culture was washed, diluted and approximately 200 cells were spread on YPD-agar plate. Randomly 95 colonies were tested for resistance to uniconazole. One colony (TJ3832) was resistant and was sequenced.

2024-04-30 | E-MTAB-14025 | biostudies-arrayexpress

H99 derived Uniconazole (1ug/mL) adaptors

Project description:Approximately 1 million cells of Crypotoccus neoformans lab strain H99 were spread on YPD-agar plate supplemented with 1 ug/mL uniconazole. After 5 days incubation of the plate at 30℃, randomly 30 adaptors were chosen and sequenced.

2024-04-30 | E-MTAB-14024 | biostudies-arrayexpress

DNA-seq of Cryptococcus neoformans H99 derived amphotericin B adaptors

Project description:Approximately 1 million cells of Cryptococcus neoformans lab strain H99 were spread on YPD plate supplmemented with 3ug/ml amphotericin B. Randomly 30 adaptors (TJ1832 - TJ1861) were chosen. The parent and all the 30 adaptors were sequenced.

2023-05-11 | E-MTAB-12493 | biostudies-arrayexpress

TJ1843 derived amphotericin B (8ug/ml) adaptors

Project description:In this project, H99 is the wild type Cryptococcus neoformans strain. TJ1843 is a strain with segmental disomy of chromosome 11. TJ1843 is a derivative of H99. Approximately 1 million cells of TJ1843 were spread on YPD plate supplemented with 8 ug/ml amphotericin B. Randomly 27 adaptors (TJ3409-TJ3435) were chosen. These adaptors were sequenced.

2023-05-13 | E-MTAB-12566 | biostudies-arrayexpress

TJ1843 derived amphotericin B (8ug/ml) adaptors

Project description:TJ1843 derived amphotericin B (8ug/ml) adaptors

| PRJEB59566 | ENA

Cryptococcus neoformans strain TJ1854 derived amphotericin B adaptors

Project description:In this study, H99 is a wild type Cryptococcus neoformans strain. TJ1854 is a chromosome 4 disomy strain derived from H99. Approximately 1 million cells of TJ1854 were spread on YPD plate supplemented with 6ug/ml amphtericin B. Randomlyh 27 adaptors (TJ2487-TJ2513) were chosen. These adaptors were sequenced.

2023-03-15 | E-MTAB-12480 | biostudies-arrayexpress

Cryptococcus neoformans TJ1843 DNA-seq derived fluconazole adaptors

Project description:In Cryptococcus neoformans, H99 is the wild type. TJ1843 is a strains with segmental disomy of chromosome 11, which is derived from H99. In this study, approximately 1 million cells of TJ1843 were spread on YPD plate supplemented with 32 ug/ml fluconazole. Randomly 27 adaptors (TJ3305 and TJ3331) were chosen. These adaptors were sequenced.

2023-08-17 | E-MTAB-12540 | biostudies-arrayexpress

TJ1854 derived flucnaozle adaptors

Project description:In Cryptococcus neoformans, H99 is the wild type. TJ1854 is the chromosome 4 disomy strain dervied from H99. In this study, approximately 1 million cells of TJ1854 were spread on YPD plate supplemented with 32ug/ml fluconazole. Randomly 27 adaptors (TJ3249-TJ3275) were chosen. These adaptors were sequenced.

2023-08-25 | E-MTAB-12478 | biostudies-arrayexpress

DNA-seq of Cryptococcus neoformans H99 derived amphotericin B adaptors

Project description:DNA-seq of Cryptococcus neoformans H99 derived amphotericin B adaptors

| PRJEB58835 | ENA