Immigration Experiment
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB75447 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Microbial analysis is challenged by immigration
| PRJEB15624 | ENA
Project description:Wastewater treatment plants use a variety of bioreactor types and configurations to remove organic matter and nutrients. Little is known regarding the effects of different configurations and within-plant immigration on microbial community dynamics. Previously, we found that the structure of ammonia-oxidizing bacterial (AOB) communities in a full-scale dispersed growth activated sludge bioreactor correlated strongly with levels of NO2- entering the reactor from an upstream trickling filter (Wells et al 2009). Here, to further examine this puzzling association, we profile within-plant microbial biogeography (spatial variation) and test the hypothesis that substantial microbial immigration occurs along a transect (raw influent, trickling filter biofilm, trickling filter effluent, and activated sludge) at the same full-scale wastewater treatment plant. AOB amoA gene abundance increased >30-fold between influent and trickling filter effluent concomitant with NO2- production, indicating unexpected growth and activity of AOB within the trickling filter. Nitrosomonas europaea was the dominant AOB phylotype in trickling filter biofilm and effluent, while a distinct ‘Nitrosomonas-like’ lineage dominated in activated sludge. Prior time series indicated that this ‘Nitrosomonas-like’ lineage was dominant when NO2- levels in the trickling filter effluent (i.e., activated sludge influent) were low, while N. europaea became dominant in the activated sludge when NO2- levels were high. This is consistent with the hypothesis that NO2- production may co-occur with biofilm sloughing, releasing N. europaea from the trickling filter into the activated sludge bioreactor. Phylogenetic microarray (PhyloChip) analyses revealed significant spatial variation in taxonomic diversity, including a large excess of methanogens in the trickling filter relative to activated sludge and attenuation of Enterobacteriaceae across the transect, and demonstrated transport of a highly diverse microbial community via the trickling filter effluent to the activated sludge bioreactor. Our results provide compelling evidence that substantial immigration between coupled process units occurs and may exert significant influence over microbial community dynamics within staged bioreactors.
2015-11-03 | GSE52079 | GEO
Project description:T cells are essentially involved in safeguarding homeostasis through fighting against the pathogens and malignant cells. T cell immunodeficiency, especially their perturbation in the severe infection, irradiation, chemotherapy, and thymic atrophy in ageing, is detrimental. Therefore, strategies that enhance T cell reconstitution provide considerable benefit and warrant intensive investigation. Here, we constructed a T cells ablation model in Tg(coro1a:DenNTR) zebrafish via administrating a proper volume of metronidazole (MTZ). T cells completely recovered at 6.5 days post treatment (dpt). The nascent regenerated T cells were mainly derived from the immigration of hematopoietic stem/progenitor cells (HSPCs) in the kidney, the functional homologue of BM. cxcr4b, but not ccr9 nor ccr7, was drastically unregulated in the responsive HSPCs. Functional interference of CXCR4 via both genetic and chemical assays yielded limited influence in T lymphopoiesis but notably delayed T cells regeneration by a destroyed HSPCs migration. In contrast, hematopoietic providing cxcr4b in Tg:(coro1a:cxcr4b) accelerates thymus-replenishment of HSPCs. Correspondingly, Cxcl12b, the ligand of Cxcr4, exhibited impressive increment presentation in the thymic epithelial cells of injured animals. Interfering or overacting Cxcl12b in either cxcl12b-/- mutants or Tg(hsp70:cxcl12b) recapitulated the similarly compromised or promoted T cells recovery as that seen in Cxcr4b scenario. Therefore, CXCR4-CXCL12 axis plays a crucial role in promoting thymocyte reconstitution but did not influence T cell development. Our study discloses a special role of CXCR4-CXCL12 signaling in promoting T cells recovery and provides a promising target to mitigate T cell immunodeficiency.
2022-08-29 | GSE190921 | GEO
Project description:Heterogous hybridization experiment
| PRJNA88017 | ENA