Project description:Using 4 replicate males and 4 replicate females this experiment examined dosage compensation and sex-biased gene expression. Briefly we performed a de novo assembly of the Manduca sexta transcriptome using all sequenced libraries, quantified genes expression, identified the physical locations of genes through orthology to the moth Bombyx mori and examine expression differences between autosomal and Z-linked genes between males and females. Further, we examined sex-biased gene expression using the replicated data.
Project description:Vestimentiferan tubeworms are representative inhabitants of deep-sea chemosynthetic ecosystems. The plume serves as the primary organ in these invertebrates without mouths and guts, facilitating direct metabolic exchange with their surrounding environments. In this study, we present a single-cell transcriptome atlas of Paraescarpia echinospica plume and this study is the initial single-cell transcriptome sequencing for the plume of the deep-sea tubeworm. We annotated six cell clusters including hemocytes, proliferative cells, muscle cells, epithelial cells, nerve1 cells, nerve2 cells and profiled genes involved in immunity and transport. This work will provide a foundation for further studies of tubeworm at the single-cell level.
Project description:Vestimentiferan tubeworms are representative inhabitants of deep-sea chemosynthetic ecosystems. The plume serves as the primary organ in these invertebrates without mouths and guts, facilitating direct metabolic exchange with their surrounding environments. In this study, we present a single-cell transcriptome atlas of Paraescarpia echinospica plume and this study is the initial single-cell transcriptome sequencing for the plume of the deep-sea tubeworm. We annotated six cell clusters including hemocytes, proliferative cells, muscle cells, epithelial cells, nerve1 cells, nerve2 cells and profiled genes involved in immunity and transport. This work will provide a foundation for further studies of tubeworm at the single-cell level.
