Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Extradiol dioxygenase pyrosequencing

ABSTRACT: Extradiol dioxygenase pyrosequencing- Secondary successional trajectories of structural and catabolic bacterial communities in oil-polluted soil planted with hybrid Poplar

PROVIDER: PRJEB7647 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR664739.fastq.gz	Fastqsanger.gz
	ERR664740.fastq.gz	Fastqsanger.gz
	ERR664741.fastq.gz	Fastqsanger.gz
	ERR664742.fastq.gz	Fastqsanger.gz
	ERR664743.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 34

Similar Datasets

Alk B pyrosequencing

Project description:Alk B pyrosequencing -Secondary successional trajectories of structural and catabolic bacterial communities in oil-polluted soil planted with hybrid Poplar

| PRJEB7645 | ENA

16S rRNA gene pyrosequnecing

Project description:16S rRNA gene pyrosequnecing- Secondary successional trajectories of structural and catabolic bacterial communities in oil-polluted soil planted with hybrid Poplar

| PRJEB7646 | ENA

Soil pyrosequencing

Project description:Soil pyrosequencing Raw sequence reads

| PRJNA422734 | ENA

Synthetic lethality by TET-dioxygenase inhibition

Project description:Synthetic lethality by TET-dioxygenase inhibition

| PRJNA682029 | ENA

Identification of indoleamine 2, 3-dioxygenase 1 (IDO1) regulated genes in human glioblastoma cell line U87

Project description:Transcriptome analysis of U87 cells under different treatments to identify IDO1-regulated genes Indoleamine 2, 3-dioxygenase 1 (IDO1) is a tryptophan (Trp) catabolic enzyme that converts Trp into downstream kynurinine (Kyn). Many studies have indicated that IDO1 is a critical suppressive immune checkpoint molecule invovled in various types of cancer. Canonically, the underlying mechanism of IDO1 immunosuppressive role is related with its enzyme activity, that is the depletion of Trp and accumulation of Kyn lead to increased tumor infiltrating suppressive regulatory T cells. Recent studies, however, challenged this hypothesis and imply that tumor cell-derived IDO1 can mediate immunosuppression independent of its enzyme activity. In this study, we aim to identify genes that are regulated by IDO1 in human glioblastoma cells, a gene expression regulatory function of IDO1 that is indepent of its enzyme activity.

2021-12-10 | GSE175700 | GEO

Rock coating pyrosequencing

Project description:Rock coating pyrosequencing reads from samples collected in Sweden

| PRJEB5935 | ENA

Synthetic lethality by TET-dioxygenase inhibition

Project description:To characterize target specificity of TETi76, we performed global gene expression analyses of K562TET2+/+ and TET2-/- control cells; TETi76 mimicked expression signatures generated by the loss of TET2 in K562. The addition of Ascorbic Acid (AA), known to enhance TET-dioxygenase activity, counteracted the changes induced by TETi76. In order to study the molecular pathway of synthetic lethality by TET-dioxygenase inhibition, natural TET2-/- mutant cell line SIGM5 was treated with TET inhibitor TETi76 and global gene expression analysis was performed by RNAseq. Result demostrate a significant upregulation of TNT-α signaling and the down regulation of Interferon-α signaling. Interestingly, we also observed significant up-modulation of oxidative stress response pathway genes consistent with the inhibition of dioxygenases. In particular, TETi76 treatment induces 8-fold increase of oxidative stress sensor NQO1 a NRF2 target gene that has been shown earlier to induce pro-apoptotic cell death in cancer cells.

2020-12-03 | GSE162487 | GEO

Muscle regeneration controlled by a designated DNA dioxygenase

Project description:Muscle regeneration controlled by a designated DNA dioxygenase

| PRJNA666123 | ENA

High-throughput 16S rRNA amplicon pyrosequencing of contaminated aquifer sediment

Project description:Contaminated aquifer (Dusseldorf-Flinger, Germany) templates extracted from 5 sediment depths ranging between 6.4 and 8.4 m below ground and over 3 years of sampling were amplified for amplicon pyrosequencing using the primers Ba27f (5’-aga gtt tga tcm tgg ctc ag-3’) and Ba519r (5’- tat tac cgc ggc kgc tg-3’), extended as amplicon fusion primers with respective primer A or B adapters, key sequence and multiplex identifiers (MID) as recommended by 454/Roche. Amplicons were purified and pooled as specified by the manufacturer. Emulsion PCR (emPCR), purification of DNA-enriched beads and sequencing run were performed following protocols and using a 2nd generation pyrosequencer (454 GS FLX Titanium, Roche) as recommended by the developer. Quality filtering of the pyrosequencing reads was performed using the automatic amplicon pipeline of the GS Run Processor (Roche), with a slight modification concerning the valley filter (vfScanAllFlows false instead of TiOnly) to extract the sequences. Demultiplexed raw reads were furhter trimmed for quality and lenght (>250 bp).

2012-11-11 | GSE25345 | GEO

Pyrosequencing of soil 16S rRNA gene

Project description:Pyrosequencing analysis of Soil Consortia Degrading Octachlorodibenzofuran

| PRJEB4603 | ENA