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DdLinBien

ABSTRACT: Linum bienne (pale flax)

PROVIDER: PRJEB76769 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR13317830_1.fastq.gz	Fastqsanger.gz
	ERR13317830_2.fastq.gz	Fastqsanger.gz
	ERR13304154.fastq.gz	Fastqsanger.gz

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Expression analysis of Linum usitatissimum 25-mers and 60-mers arrays

2012-05-15 | GSE37980 | GEO

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Project description:Linum bienne (pale flax), genomic and transcriptomic data

| PRJEB76768 | ENA

Expression analysis of Linum usitatissimum 25-mers and 60-mers arrays

Project description:Performances of flax gene expression analyses were compared in two categories of Nimblegen microarrays (short 25-mers oligonucleotides and long 60-mers oligonucleotides) Results obtained in this study are described in Intra-platform comparison of flax (Linum usitatissimum L.) high-density Nimblegen DNA microarrays submitted to Journal of Computational Biology We compared two categories of flax target probes: short (25-mers) oligonucleotides and long (60-mers) oligonucleotides in identical conditions of target production, design, labelling, hybridization, image analyses, and data filtering. This comparison was realized with two different flax samples and each RNA sample was used for the two categories of arrays. Experiments were realized in order to discriminate specific gene expression profiles of two different flax tissues (outer and inner stem tissues).

2012-05-14 | E-GEOD-37980 | biostudies-arrayexpress

Whole-genome DNA methylation landscapes of control and heat-stressed Linum usitatissimum seedlings.

Project description:Whole-genome bisulfite sequencing (WGBS) was employed for identification of differential DNA methylation profiles among control and heat-stressed seedlings of a fibre flax (Linum usitatissimum L.) var., JRF-2. It was identified as a tolerant variety of heat stress-induced oxidative damage. High-quality genomic DNA from four samples comprised 3-week-old control and heat-stressed (40±2°C) seedlings, with or without treated with 5-Azacytidine (hypomethylating agent). High-quality and filtered paired-end Illumina reads were aligned to the flax reference genome, assembled in chromosomes, using bwa-meth tool, followed by methylation loci (5-mC) calling using the MethylDackel software. Differentially methylated regions (DMRs) between the control and other samples were identified using the methylKit and annotated using genomation package for their precedence in the promoter/exon/intron/intergenic regions. The DMRs comprised both hyper- and hypomethylated loci, but the latter found dominated due to heat stress in flax seedlings. The WGBS in flax for heat stress will provide a platform to identify epigenetic loci responsible for heat-stress adaptation in flax.

2022-04-01 | GSE185571 | GEO

Mass spectrometric identification of antimicrobial peptides from medicinal seeds

Project description:Traditional medicinal plants contain a variety of bioactive natural products including cysteine-rich (Cys-rich) antimicrobial peptides (AMPs). Cys-rich AMPs are often crosslinked by multiple disulfide bonds which increase their resistance to chemical and enzymatic degradation. However, this class of molecules is relatively underexplored. Herein, in silico analysis predicted 80 – 100 Cys-rich AMPs per species from three edible traditional medicinal plants: Linum usitatissimum (flax), Trifolium pratense (red clover), and Sesamum indicum (sesame). Proteomics analysis of seed peptide extracts revealed direct evidence for the translation of 3-10 Cys-rich AMPs per species including lipid transfer proteins, defensins, α-hairpinins, and snakins. Negative activity revealed by antibacterial screening highlights the importance of employing a multi-pronged approach for AMP discovery. Further, this study demonstrates that flax, red clover, and sesame are promising sources of further AMP discovery and characterization.

2022-02-16 | PXD029289 | Pride

Project description:Transcriptome of flax (Linum usitatissimum) seedlings after Fusarium oxysporum treatment

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Flax rust effector transient expression in flax leaves RNA seq

Project description:To investigate theinfluence of Flax rust effectors on gene expression when transiently overexpressed in flax leaves.

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Project description:Transcriptome of flax (Linum usitatissimum) seedlings after 0,5 mT EMF treatment

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Transcriptome of flax (Linum usitatissimum) seedlings after 100 uM ABA treatment

Project description:Transcriptome of flax (Linum usitatissimum) seedlings after 100 uM ABA treatment

| PRJNA515812 | ENA

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