Project description:In this study, whole blood samples were used to determine the gene expression of febrile culture confirmed enteric fever cases (ST = S. Typhi; SPT = S. Paratyphi), febrile culture negative individuals presenting to hospital in Kathamandu (sEF = suspected enteric fever), and healthy community controls (CTRL).
Project description:Chronic pruritus is a pathological condition associated with numerous systemic, dermatologic, and neurologic disorders. Chronic pruritus of unknown origin (CPUO) represents a distinct clinical subtype characterized by persistent itch without an identifiable underlying cause. While emerging evidence implicates neuroimmune dysregulation in chronic pruritus, the specific mechanisms driving CPUO remain poorly understood. Objective: To characterize the neuroimmune mechanisms underlying CPUO, with a focus on identifying potential diagnostic markers. Single-cell RNA sequencing of peripheral blood cells from patients and healthy controls was conducted. Single-cell transcriptomic analysis revealed dysregulated monocytes and hyperactive natural killer (NK) cells overexpressing CCL3.
