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The modulatory effect of visfatin (NAMPT) on the transcriptomic profile of porcine anterior pituitary cells during the peri-implantation period

ABSTRACT: The modulatory effect of visfatin (NAMPT) on the transcriptomic profile of porcine anterior pituitary cells during the peri-implantation period

PROVIDER: PRJEB79001 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR13491700_1.fastq.gz	Fastqsanger.gz
	ERR13491700_2.fastq.gz	Fastqsanger.gz
	ERR13491701_1.fastq.gz	Fastqsanger.gz
	ERR13491701_2.fastq.gz	Fastqsanger.gz
	ERR13491702_1.fastq.gz	Fastqsanger.gz

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The modulatory effect of visfatin (NAMPT) on the transcriptomic profile of porcine anterior pituitary cells during the peri-implantation period

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VISFATIN (NAMPT) AFFECTS GLOBAL GENE EXPRESSION IN PORCINE ANTERIOR PITUITARY CELLS DURING THE MID-LUTEAL PHASE OF THE OESTROUS CYCLE

Project description:Pituitary belongs to a most important endocrine glands which takes part in the regulation of reproductive functions. The proper functioning of this intermediary between the central nervous system and the target tissues of the reproductive system ensures the proper course of the estrous cycle and affects the female's reproductive potential. It is believed that visfatin, a hormone belonging to the adipokine family, may be responsible for the control of reproductive functions in response to the actual metabolic state of female. Herein we verified hypothesis assuming the modulatory effect of visfatin on the anterior pituitary transcriptome during the mid-luteal phase of the oestrous cycle. We analyzed samples obtained from 5 individuals (n=5). After the enzymatic tissue digestion with the use collagenase V and pancreatin cells were divided for two groups: controls [without treatment] and visfatin (100 ng/mL) treated ones. In vitro cell cultures were conducted for 24 hours.

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Project description:In pigs, the peri-implantation period is recognized as determinant of successful reproduction, and it is controlled by neuroendocrinal interactions among signals originating from the female hypothalamic-pituitary-ovarian axis, uterus, and developing embryo. The transcriptomic activity of the pituitary gland may influence reproductive processes occurring in pigs during the peri-implantation period. The aim of this study was to determine alternations in gene expression in the porcine pituitary gland during the peri-implantation period (days 15-16 of pregnancy) in comparison with that during the respective days of the estrous cycle using a microarray approach.

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VISFATIN (NAMPT) AFFECTS GLOBAL GENE EXPRESSION IN PORCINE ANTERIOR PITUITARY CELLS DURING THE MID-LUTEAL PHASE OF THE OESTROUS CYCLE

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The role of nicotinamide phosphoribosyltransferase (Nampt) in hippocampal neural stem/progenitor cells

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