Grass Phylogeny Working Group III
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB79360 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| ERR13764200.fastq.gz | Fastqsanger.gz | |||
| ERR13764300.fastq.gz | Fastqsanger.gz | |||
| ERR13764400.fastq.gz | Fastqsanger.gz | |||
| ERR13764500_1.fastq.gz | Fastqsanger.gz | |||
| ERR13764500_2.fastq.gz | Fastqsanger.gz |
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Project description:SERPINA1, a member of the serine protease inhibitor family, plays a role in viral infection and inflammation by regulating the activities of serine and cysteine proteases. To further investigate the antiviral role SERPINA1 played in GCRV (Grass carp Reovirus) infection, a polyclonal antibody of SERPINA1 was prepared, and the protein interacting with SERPINA1 was screened by CoIP/MS in grass carp hepatopancreas tissue. Samples of hepatopancreas tissues from grass carp (n=3) before (healthy) and 8 days after the infection (post-infection) were selected. Grass carp were infected by intraperitoneal injection. The total tissue proteins were extracted according to the manufacturer’s instructions for cell lysate (Beyotime, Shanghai, China). The types and abundance of proteins bound to SERPINA1 before and after the infection were detected and analyzed using CoIP-MS.
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