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Genome-wide mapping of HIST1H2ac binding sites in MCF-7 cells

ABSTRACT: Genome-wide mapping of HIST1H2ac binding sites in MCF-7 cells

PROVIDER: PRJEB7978 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	ERR700054.fastq.gz	Fastqsanger.gz
	ERR700055.fastq.gz	Fastqsanger.gz
	ERR700056.fastq.gz	Fastqsanger.gz
	ERR700057.fastq.gz	Fastqsanger.gz
	ERR700058.fastq.gz	Fastqsanger.gz

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Project description:Previously, we have shown that HIST1H2ac is overexpressed in MCF-7 breast cancer cell line. It acts as a master regulator of estrogen receptor alpha-dependent gene expression in ER+ breast cancer cells. In the present study, we investigate the genome-wide protein DNA-binding events of HIST1H2ac protein in MCF-7 breast cancer cell line by over-expressing hemagglutinin (HA)-tagged HIST1H2ac and compared with MCF-7 cells over-expressing HA. The protein-bound DNA was recovered by immunoprecipitation using anti-HA antibody. The ChIP DNA and input DNA were sequenced with an Illumina HiSeq 2000 sequencer.

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