XgAniVori1
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB80127 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Anisus vorticulus (little whirlpool ram's-horn snail)
| PRJEB77487 | ENA
Project description:Anisus vorticulus (little whirlpool ram's-horn snail) genome assembly, xgAniVori1, alternate haplotype
| PRJEB80126 | ENA
Project description:Anisus vorticulus (little whirlpool ram's-horn snail), genomic and transcriptomic data
| PRJEB77486 | ENA
Project description:Anisus vortex (whirlpool ramshorn) genome assembly, xgAniVort1
| PRJEB59452 | ENA
Project description:Anisus vortex (whirlpool ramshorn) genome assembly, xgAniVort1, alternate haplotype
| PRJEB59451 | ENA
Project description:Anisus vortex (whirlpool ramshorn), genomic and transcriptomic data
| PRJEB59082 | ENA
Project description:Immediate early genes (IEGs) are useful markers of neuronal activation and essential components of neuronal response. While studies of gastropods have provided many insights into the basic learning and memory mechanisms, the genome-wide assessment of IEGs has been mainly restricted to vertebrates. In this study we identified IEGs in the terrestrial snail Helix lucorum. In the absence of the genome, we conducted de novo transcriptome assembly using reads with short and intermediate lengths cumulatively covering more than 98 billion nucleotides. Based on this assembly, we identified 37 proteins corresponding to contigs differentially expressed in either the parietal ganglia or two giant interneurons located within the parietal ganglia of the snail in response to the neuronal stimulation. These proteins included homologues of well-known mammalian IEGs, such as c-jun/jund, C/EBP, c-fos/fosl2 and Egr1, as well as homologues of genes not yet implicated in the neuronal response.
2019-05-14 | GSE123558 | GEO
Project description:The dorsal horn of the spinal cord transforms incoming somatosensory information and transmits it supraspinally to generate sensory perception, including pain and itch. Recent research using mouse Cre-driver lines has implicated specific populations of dorsal horn neurons in the transmission of different types of pain. In parallel, human genome-wide association studies (GWAS) have identified dozens of loci confidently associated with the genetic predisposition to chronic pain. The ability to connect controlled experiments in rodent models with human genetic studies could provide a platform for translational research, but the cell type heterogeneity of the dorsal horn and the complex genetic architecture of chronic pain have created challenges in bridging that gap. Here, we apply a variety of single cell genomic technologies and a comparative genomic analysis to identify conserved dorsal horn neuron subtypes whose open chromatin regions show enrichment for genetic variants associated with human chronic pain phenotypes. To achieve this, we first use single nucleus RNA-Seq and fluorescence in situ hybridization in Rhesus macaque to create a more detailed map of primate dorsal horn neuron subtypes. These were integrated with publicly available human and mouse single nucleus RNA-Seq datasets to create a multi-modal cross species atlas. Then, for the mouse dorsal horn, we combined single nucleus RNA-Seq, spatial transcriptomics, and single nucleus ATAC-Seq to infer spatial and epigenomic profiles of conserved dorsal horn neuron subtypes. Finally, we compared our conserved cell-type open chromatin resource to chronic pain GWAS and found that open chromatin regions of specific dorsal horn neuron subtypes showed enrichment for a variety of human chronic pain phenotypes. Our results provide a foundation to further explore how conserved dorsal horn neuron subtypes influence the transmission of pain signals.
2024-08-01 | GSE253952 | GEO