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IcPhaCoch

ABSTRACT: Phaedon cochleariae (mustard beetle)

PROVIDER: PRJEB80146 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR13698308.fastq.gz	Fastqsanger.gz
	ERR13670066_1.fastq.gz	Fastqsanger.gz
	ERR13670066_2.fastq.gz	Fastqsanger.gz

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icPhaCoch

Project description:Phaedon cochleariae (mustard beetle), genomic and transcriptomic data

| PRJEB80145 | ENA

Interaction of Chinese cabbage (Brassica rapa ssp. pekinensis) cell wall proteins with mustard leaf beetle (Phaedon cochleariae) polygalacturonase family members

Project description:Plants possess various defense strategies to counter attacks from microorganisms or herbivores. For example, plants reduce the cell-wall-macerating activity of pathogen- or insect-derived polygalacturonases (PGs) by expressing PG-inhibiting proteins (PGIPs). PGs and PGIPs belong to multi-gene families believed to have been shaped by an evolutionary arms race. The mustard leaf beetle Phaedon cochleariae expresses both active PGs and catalytically inactive PG pseudoenzymes. Previous studies demonstrated that (i) PGIPs target beetle PGs and (ii) the role of PG pseudoenzymes remains elusive, despite having been linked to the pectin degradation pathway. For further insight into the interaction between plant PGIPs and beetle PG family members, we combined affinity purification with proteomics and gene expression analyses, and identified novel inhibitors of beetle PGs from Chinese cabbage (Brassica rapa ssp. pekinensis). A beetle PG pseudoenzyme was not targeted by PGIPs, but instead interacted with PGIP-like proteins. Phylogenetic analysis revealed that PGIP-like proteins clustered apart from classical PGIPs but together with proteins, which have been involved in developmental processes. Our results indicate that PGIP-like proteins represent not only interesting novel PG inhibitor candidates in addition to classical PGIPs, but also fascinating new players in the arms race between herbivorous beetles and plant defenses.

2021-06-07 | PXD022562 | Pride

Phaedon cochleariae

Project description:Phaedon cochleariae overview

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PCO-RNAi

Project description:Phaedon cochleariae RNAi knockdown of GH28

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Phaedon cochleariae

Project description:Phaedon cochleariae Transcriptome or Gene expression

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icPhaTumi

Project description:Phaedon tumidulus (celery leaf beetle)

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PGI:Phaedon cochleariae genome

Project description:Phaedon cochleariae genome and annotation from the Pest Genomics Initiative.

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icPhaTumi

Project description:Phaedon tumidulus (celery leaf beetle), genomic and transcriptomic data

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Unique metabolism of different glucosinolates in larvae and adults of a leaf beetle specialised on Brassicaceae

Project description:Brassicaceae plants contain glucosinolates, which are hydrolysed by myrosinases to toxic products such as isothiocyanates and nitriles, acting as defences. Herbivores have evolved various detoxification strategies, which are reviewed here. Larvae of Phaedon cochleariae (Coleoptera: Chrysomelidae) metabolise hydrolysis products of benzenic glucosinolates by conjugation with aspartic acid. In this study, we investigated whether P. cochleariae uses the same metabolic pathway for structurally different glucosinolates, whether the metabolism differs between adults and larvae and which hydrolysis products are formed as intermediates. Feeding experiments were performed with leaves of watercress (Nasturtium officinale, Brassicaceae) and pea (Pisum sativum, non-Brassicaceae), to which glucosinolates with structurally different side chains (benzenic, indole or aliphatic) or their hydrolysis products were applied. Samples were analysed by UHPLC-QTOF-MS/MS or TD-GC-MS. The same aspartic acid conjugates as previously identified in larvae were also detected as major metabolites of benzenic glucosinolates in adults. Indol-3-ylmethyl glucosinolate was mainly metabolised to N-(1H-indol-3-ylcarbonyl) glutamic acid in adults and larvae, while the metabolism of 2-propenyl glucosinolate remains unclear. The metabolism may thus proceed primarily via isothiocyanates rather than via nitriles, while the hydrolysis occurs independently of plant myrosinases. A detoxification by conjugation with these amino acids is not yet known from other Brassicaceae-feeders.

2022-11-11 | MTBLS3565 | MetaboLights

Expression profiling of Solanum chacoense in response to Colorado potato beetle herbivory

Project description:We report the transcriptional response to Colorado potato beetle herbivory in leaves of the highly beetle resistant Solanum chacoense diploid line USDA8380-1 (80-) and a susceptible F2 individual (EE501F2_093) derived from a cross between 80-1 and a beetle susceptible line S. chacoense M6. Sampling tissue in a time course during adult Colorado potato beetle feeding provides novel insight to the transcriptomic defense response to this important pest.

2023-03-08 | GSE169331 | GEO

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