JhMilDich
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB80500 | ENA |
REPOSITORIES: ENA
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Project description:Purpose: Corals are major sources of dimethylsulphoniopropionate (DMSP), a compound that plays a central role in the global sulphur cycle. While DMSP biosynthesis pathways have been investigated in plants and algae, the molecular basis for its production by corals is unknown. Given its potential role as an osmolyte, the effect of salinity stress on levels of DMSP was investigated in both adults and juveniles (lacking photosynthetic symbionts) of the coral Acropora millepora. This study used transcriptomic data to analyse the effects of salinity over the coral A. millepora and to identify coral genes likely to be involved in DMSP biosynthesis. Methods: Adults coral transcriptomic libraries were constructed from samples exposed during 1 and 24 hours of salinity treatment (25 PSU) and control (35 PSU) conditions (n=5 per condition). Juveniles coral transcriptomic libraries were constructed from samples exposed to 24 and 48 hours of salinity treatment (28 PSU) and control (35 PSU) conditions (n=6 per condition). All libraries were sequenced by 100 bp paired-end in a HiSeq 2000. Reads were mapped onto the Acropora millepora genome using TopHat2 to produce a count data gene expression matrix for subsequent gene expression analysis using DESeq2 package. Results: In adult coral samples, 5.5 - 10.2 million RNAseq reads were obtained for each treatment sampling time while 3.4 - 8.8 million reads were obtained for each juvenile coral sample. The count matrix of the 26,622 A. millepora gene predictions were generated using htseq-count workflow. BlastP analysis of the A. millepora gene predictions led to the identification of coral members of gene families implicated in DMSP biosynthesis in other organisms, while RNA-seq data was used to identify the differentially expressed ones in response to hyposaline stress and on this basis were considered to be candidates for roles in DMSP biosynthesis in corals. Conclusions: Hyposaline stress increased DMSP production in both adults and aposymbiotic juvenile corals, and transcriptomic analyses highlighted the potential involvement of specific candidate genes in the production of DMSP via an alga-like pathway. The biochemistry of DMSP production is not well established for any eukaryotic system and, as the first animals in which it has been demonstrated, this is particularly true in the case of corals. Our RNA-seq results enabled the identification of candidates for roles in DMSP biosynthesis in corals but, given its critical roles in diverse biological processes, a thorough investigation of the molecular mechanisms leading to its production by corals is required.
2017-08-20 | GSE96916 | GEO
Project description:We used the Illumina RNAseq approach to study the effects of acute exposure to elevated CO2 on gene expression in primary polyps of Acropora millepora Examination of transcriptome in Acropora millepora primary polyps at 380, 750 and 1000 ppm CO2 after 3 days exposure
2012-04-10 | E-GEOD-33016 | biostudies-arrayexpress
Project description:We compared the effect of acute (Moya et al 2012) and chronic exposure to elevated pCO2 on gene expression in primary polyps of Acropora millepora Examination of transcriptome in Acropora millepora primary polyps at 380 and 750 ppm CO2 (air) after 9 days exposure
2015-05-01 | E-GEOD-61114 | biostudies-arrayexpress
Project description:The aim of this study was to identify anti-obesity peptide from Allomyrina dichotoma (A. dichotoma) and investigate the biochemical signaling pathway. For that, A. dichotoma larvae was hydrolyzed enzymatically, and further purified by using tangential flow filtration and consecutive chromatographic method. Finally, anti-obesity peptide was obtained, and their sequences identified as Gln-Ile-Ala-Gln-Asp-Phe-Lys-Thr-Asp-Leu (EIA10). EIA10 prevented adipocyte differentiation in vitro and was further investigated the mechanism underlying the effects in vivo. Our results indicated that EIA10 reduced body weight gain, organ weight and adipose tissue volume. Glucose tolerance and insulin resistance in high fat diet-fed obese mice significantly improved after EIA10 administration for four weeks. In addition, EIA10 significantly decreased TC and LDL, increased HDL, improved lipid metabolism, and downregulated mRNA and protein expression of transcription factors implicated in lipid adipogenesis. Taken together our results suggest that EIA10 from possessed potential for the treatment and prevention of obesity.
2021-09-09 | PXD019661 | Pride
Project description:Gordius_albopunctatus, genomic and transcriptomic data
| PRJEB78907 | ENA