Project description:Optimisation of DNA-protein co-extraction from the thin microbial biofilm inhabiting marine plastic debris for meta-omics and comparative metaproteomics analysis.

2023-06-20 | MSV000092214 | MassIVE

Method DNA extraction Mars simulant soil

Project description:DNA extraction method for synthetic Mars soil

| PRJEB68335 | ENA

The effects from DNA extraction methods on the evaluation of microbial diversity associated with human colonic tissue

Project description:Recent advances in (meta)genomic methods have provided new opportunities to examine host-microbe-environment interactions in the human gut. While opportunities exist to extract DNA from freshly sourced colonic tissue there are potentially valuable sources of DNA from historical studies that might also be examined. We examined how four different tissue DNA extraction methods employed in past clinical trials might impact the recovery of microbial DNA from a colonic tissue sample as assessed using a custom designed phylogenetic microarray for human gut bacteria and archaebacteria. While all methods of DNA extraction produced similar phylogenetic profiles some extraction specific biases were also observed. Real time PCR analysis targeting several bacterial groups substantiated this observation. These data suggest that while the efficacy of different DNA extraction methods differs somewhat all the methods tested produce an accurate representation of microbial diversity. This suggests that DNA samples archived in biobanks should be suitable for retrospective analyses.

2010-01-01 | GSE18420 | GEO

Impact of sand extraction on sediment prokaryote communities

Project description:Impact of sand extraction on sediment prokaryote communities

| PRJEB80905 | ENA

Negative and positive control samples - DNA extraction

Project description:References samples to the DNA extraction

| PRJEB39304 | ENA