Project description:Members of the Caryophyllaceae Juss. family possess anabolic, adaptogenic, radioprotective, antitumoral and hemorheological properties and have been used in medical practice for the treatment of various inflammatory disorders. Macrophages are key immune cells that coordinate the inflammatory reaction. To identify the effects of Silene sendtneri and Silene roemeri on macrophage activation, primary human macrophages were cultured with extracts of these plants .We used microarrays to determine the global expression pattern induced by the extracts in macrophages.
Project description:ATAC-seq was used to locate putative enhancer regions in the early development (tailbud stage), developing male and female gonads of the marine chrodate Oikopleura dioica.
Project description:Under crowded, nutrient-limiting conditions, growth in the marine chordate O. dioica arrests until favorable conditions return. We profiled translation genome-wide using ribosome profiling in O. dioica during growth arrest and growth arrest recovery. We found that initial recovery is independent of nutrient-responsive, trans-spliced genes, suggesting that animal density is the primary trigger for the resumption of development in this species.
Project description:ChIP-seq was used to generate chromatin state maps, profile binding patterns of key architectural proteins and locate putative enhancer regions in the early development (TB stage) and developing gonads of the marine chrodate Oikopleura dioica.
