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ABSTRACT: 560 Large White sows whole genome sequencing

PROVIDER: PRJEB82721 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	InDel.qc.vcf.csi	Other
	InDel.qc.vcf.gz	Vcf
	SNP.qc.vcf.csi	Other
	SNP.qc.vcf.gz	Vcf
	SV.merge.qc.vcf.csi	Other

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Project description:Purpose:The goal of this study was to enrich understanding of the reproductive difference Methods: Multiparous Canadian Large White cyclic sows were divided into two parts: high (H; total number of piglets born > 15.73) and low (L; total number of piglets born < 11.11) fecundity. Eight sows with similar parity from each part were chosen (n=16). Ovarian tissues were obtained on the 14 day (day 1 = first day of estrus) after estrus as in the luteal phase (L) and 20 day of the estrous cycle as in the follicular phase (F). Transcriptome profiling of ovarian tissues were generated by deep sequencing, in quadruplicate, using Illumina HiSeq X10 instrument. Results: Using an optimized data analysis workflow, we obtained the differentially expressed miRNAs between the high and low fecundity, with a fold change ≥1.5 and p value < 0.05. These results provide further insight into fecundity in pigs. Conclusions: Our study represents the first detailed analysis of ovary transcriptome. It will be significantly helpful to display a novel regulatory mechanism for further investigation of prolificacy in pigs.

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Transcriptome profiling of ovarian tissues from pigs with extreme phenotypes for reproductive efficiency during the luteal and follicular phases of the estrous cycle

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