Project description:Arundo donax L. is one of the most promising bioenergy crop due to its high biomass yield and low irrigation requirement. The resistance to biotic and abiotic stress causes the high invasiveness of this plant, which can grow with very low management input (e.g., pesticides, fertilization, irrigation) even in marginal lands or in fields irrigated with waste or salty water. We report the leaf transcriptome sequencing, de novo assembly and annotation of a giant reed G34 genotype under salt stress. This genotype shows a different transcriptomic response to salinity compared to other A. donax genotypes. This finding was unexpected considering that the genetic variability of this species is supposed to be low due to its vegetative reproductive process. This study aims to direct future efforts towards the A. donax genetic improvement.
Project description:The objective of this project was to investigate the impacts of oil exposure in combination with environmental stressors on gene expression in developing C. variegatus.
Project description:The objective of this project was to expose larval Cyprinodon variegatus to oil/hypoxia stressors for 48 hrs, then allow 48 hours of recovery and depuration.
Project description:The adhesion of flavescence dorée phytoplasma to the midgut epithelium cells of their insect vectors is partially mediated by the Variable Membrane Protein A (VmpA), an adhesin which shows lectin properties. In order to identify the insect receptor for VmpA, we lokked for Euscelidius variegatus cells proteins interacting with recombinant VmpA-His6 by mass spectrometry analysis of VmpA-E. variegatus protein complexes formed upon in vitro interaction assays.
