Vaginal microbiota of Latxa ewes
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PROVIDER: PRJEB83796 | ENA |
REPOSITORIES: ENA
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Project description:Description of the vaginal microbiota in nulliparous ewes during natural mating and pregnancy: preliminary evidence of the male preputial microbiota influence
| PRJEB63647 | ENA
Project description:<p>Introduction: Gonadotropin-releasing hormone (GnRH) is widely used in the timed artificial insemination program of sheep industry, but there is still controversy about the effect of GnRH on pregnancy rate in the process of artificial insemination. Our research shows that the use of GnRH reduces the pregnancy rate. This study aims to analyze the metabolite changes caused by GnRH in Huyang ewes before the implantation period through metabolomics technology, and reveal the mechanism behind the decreased pregnancy rate. </p><p>Methods: All ewes had a polyurethane sponge containing 45 mg of flurogestone acetate placed in their vagina for 12 days, and were intramuscularly treated with 330 units of equine chorionic gonadotropin (eCG) immediately after sponge removal. The experimental group (n = 69) received an intramuscular treatment of 17 μg GnRH agonist triptorelin (Day 0) 48 h after sponge removal, while the control group (n = 41) was treated with 1 mL of sterile saline. All ewes underwent a single vaginal insemination 58 h after the withdrawal of progesterone sponges. The difference in pregnancy rates between the two groups was then measured. Metabolomic analysis was performed on plasma samples collected on Day 7. </p><p>Results: Compared with the control group, GnRH treatment significantly reduced the pregnancy rate in the experimental group (72.2% vs 82.9%, P < 0.05). Metabolomic analysis revealed that GnRH treatment influenced metabolites involved in collagen synthesis and metabolites linked to prostaglandin synthesis in the endometrial tissue, which includes a marked decrease in hydroxyproline metabolite content and a substantial increase in corticosterone and prostaglandin D2 metabolite levels. </p><p>Conclusion: In summary, GnRH agonist Triptorelin injected 48 h after the withdrawal of progesterone sponges can reduce the pregnancy rate of Huyang ewe after artificial insemination and affect the metabolite levels related to endometrial collagen synthesis and prostaglandin synthesis, which are not conducive to embryo implantation.</p>
2024-08-12 | MTBLS10318 | MetaboLights
Project description:Vaginal microbiome during and after pregnancy
| PRJNA766806 | ENA
Project description:16S profiling of the vaginal microbiome in Brucella-vaccinated pregnant ewes
| PRJEB97484 | ENA
Project description:Hormonal protocols for cervix dilation are essential for non-surgical embryo recovery (NSER) in ewes, due to the cervical complexity of the species. However, little is known about the possible effects of these hormones on the uterine environment. Thus, this study aimed to evaluate in sheep the effect of a hormonal protocol for cervical dilation on the protein profile of the uterine fluid. After superovulation, Santa Inês ewes (n = 40) were allocated into either a treated group with estradiol benzoate (100 µg, iv) and sodium cloprostenol (0.12 mg, im) 12 h before the embryo collection plus oxytocin (100 IU, iv) 15 min before the collection procedure; or a control group, which consisted of the administration of saline solution. The recovery of the uterine lavage and embryos was performed by laparotomy. After precipitation (trichloroacetic acid) and digestion (trypsin), proteins were identified by mass spectrometry (LC-MS/MS). Overall, 1447 proteins were identified, of which 436 and 126 were found exclusively in the treated and control groups, respectively. A total of 16 proteins were highly expressed in the treated group (P < 0.05), which included glutathione transferase (related to prostaglandin biosynthesis) and osteopontin (involved in the regulation of estradiol). In the control group, five proteins were up-regulated (P < 0.05), including cofilin (linked to up-regulation of embryonic development). Based on the gene ontology, in both groups, the proteins most abundantly in the uterine fluid are related to cellular and metabolic processes, in addition to locomotion and localization in the control group. In conclusion, the hormonal protocol for cervical dilation modifies the protein profile of uterine lavage recovered from superovulated ewes after embryo collection.
2025-11-14 | PXD039757 | Pride
Project description:Vaginal Microbiota Dynamics in Pregnancy
| PRJEB77434 | ENA
Project description:In the current study, 5 multiparous ewes during the late gestation were restricted to 30% feeding level to establish an undernutrition model while another 5 ewes were fed normally as controls. All the ewes were sacrificed and pituitary samples were collected to do transcriptome sequencing to investigate the metabolic changes.
2023-11-14 | GSE220723 | GEO
Project description:Clinical treatment protocols for infertility with in vitro fertilization-embryo transfer (IVF-ET) provide a unique opportunity to assess the human vaginal microbiome in defined hormonal milieu. Herein, we have investigated the association of circulating ovarian-derived estradiol (E2) and progesterone (P4) concentrations to the vaginal microbiome. Thirty IVF-ET patients were enrolled in this study, after informed consent. Blood was drawn at four time points during the IVF-ET procedure. In addition, if a pregnancy resulted, blood was drawn at 4-to-6 weeks of gestation. The serum concentrations of E2 and P4 were measured. Vaginal swabs were obtained in different hormonal milieu. Two independent genome-based technologies (and the second assayed in two different ways) were employed to identify the vaginal microbes. The vaginal microbiome underwent a transition with a decrease in E2 (and/or a decrease in P4). Novel bacteria were found in the vagina of 33% of the women undergoing IVF-ET. Our approach has enabled the discovery of novel, previously unidentified bacterial species in the human vagina in different hormonal milieu. While the relationship of hormone concentration and vaginal microbes was found to be complex, the data support a shift in the microbiome of the human vagina during IVF-ET therapy using standard protocols. The data also set the foundation for further studies examining correlations between IVF-ET outcome and the vaginal microbiome within a larger study population.
2011-10-24 | E-MEXP-2958 | biostudies-arrayexpress