Project description:We constructed three small RNA libraries from embryos at 5, 6 and 7 post-oviposition (hpo) of Bactrocera dorsalis for deep sequencing. The data analysis revealed 147 known and 103 novel miRNAs from these libraries.
Project description:We constructed four small RNA libraries from whole body of females, males (except ovaries and testes) and ovaries, testes of B. dorsalis for deep sequencing. The data analysis revealed 314 known and 221 novel miRNAs from these libraries. 14 female-biased and 12 male-biased miRNAs that may be involved in sexual differentiation were found by comparing the miRNA expression profiles in the four libraries.
Project description:Analyses of new genomic, transcriptomic or proteomic data commonly result in trashing many unidentified data escaping the ‘canonical’ DNA-RNA-protein scheme. Testing systematic exchanges of nucleotides over long stretches produces inversed RNA pieces (here named “swinger” RNA) differing from their template DNA. These may explain some trashed data. Here analyses of genomic, transcriptomic and proteomic data of the pathogenic Tropheryma whipplei according to canonical genomic, transcriptomic and translational 'rules' resulted in trashing 58.9% of DNA, 37.7% RNA and about 85% of mass spectra (corresponding to peptides). In the trash, we found numerous DNA/RNA fragments compatible with “swinger” polymerization. Genomic sequences covered by «swinger» DNA and RNA are 3X more frequent than expected by chance and explained 12.4 and 20.8% of the rejected DNA and RNA sequences, respectively. As for peptides, several match with “swinger” RNAs, including some chimera, translated from both regular, and «swinger» transcripts, notably for ribosomal RNAs. Congruence of DNA, RNA and peptides resulting from the same swinging process suggest that systematic nucleotide exchanges increase coding potential, and may add to evolutionary diversification of bacterial populations.
Project description:Comprehensive RNA sequencing was performed on a laboratory colony of B. dorsalis with a focus on attempting to capture as many genes in the sequencing from throughout the entire developmental life history. De novo assembly and analysis of the resulting sequence One sample each for the egg, larvae, pupae, adult male, adult female and mated female life stages was sequenced.
