Project description:We cultured D. immitis L3 and L4 of two laboratorial strains with different susceptibility status to macrocyclic lactone drugs in vitro. Excretory/secretory microRNAs were sequenced and analyzed.
Project description:Sheep (N=6) infested with Psoroptes ovis mites were bled weekly and circulating leukocytes isolated from their whole blood samples. RNA was extracted and processed onto Agilent Bovine Microarrays and differentially expressed genes identified between the following time points (0 (Baseline (pre-infestation)), 1 week, 3 weeks and 6 weeks post-infestation).
