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IlComBaju

ABSTRACT: Comibaena bajularia (blotched emerald), genomic and transcriptomic data

PROVIDER: PRJEB85369 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	ERR14242279_1.fastq.gz	Fastqsanger.gz
	ERR14242279_2.fastq.gz	Fastqsanger.gz
	ERR14231580.fastq.gz	Fastqsanger.gz
	ERR14242280_1.fastq.gz	Fastqsanger.gz
	ERR14242280_2.fastq.gz	Fastqsanger.gz

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ilComBaju

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Activation of an endogenous pararetrovirus, petunia vein clearing virus, during long-term cultivation of host plants is associated with disturbance of star-type floral color pattern in Petunia hybrida

Project description:White areas of star-type bicolor petals of petunia (Petunia hybrida) are caused by post-transcriptional gene silencing (PTGS) of the key enzyme of anthocyanin biosynthesis. We observed blotched flowers and vein-clearing symptom in leaves in 3- to 4-month-old petunia plants. In order to determine the cause of blotched flowers, we examined an endogenous pararetrovirus, petunia vein clearing virus (PVCV), because this virus causes vein-clearing symptom and may have a suppressor of PTGS. Transcripts and episomal DNA derived from proviral PVCV loci highly accumulated in 3- to 4-month-old plants, indicating that PVCV was activated as the host plant ages. Furthermore, CG and CHG sites of the promoter region of PVCV were highly methylated in 1-month-old plants but these cytosines were not methylated in 3- to 4-month-old plants, suggesting that poor maintenance of DNA methylation activates propagation of PVCV. In parallel, de novo methylation at CHH sites and 24-nt small RNAs were detected on the promoter region of PVCV, indicating that RNA-directed DNA methylation was induced by PVCV activation. Detections of transcripts and episomal DNA of PVCV in blotched regions and suppressor activity of PTGS support the mechanism that suppression of PTGS by activated PVCV causes blotched flowers.

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Project description:Transcriptome data of Blotched Snakehead (Channa maculata)

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Integrative analysis of green ash phloem transcripts and proteins during an emerald ash borer infestation

Project description:We performed RNA-seq and proteomics on naturally infested green ash (F. pennsylvanica) trees at low, medium and high levels of increasing emerald ash borer (A. planipennis) infestation. Our integrative analysis of the RNA-Seq and proteomics data identified 14 proteins and 4 transcripts that contribute most to the difference between highly infested and low infested trees.

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